Bioprospecção e caracterização de bactérias produtoras de ciclodextrina glicosiltranferase em solos de biomas brasileiros

• Ano de defesa: 2014
• Autor(a) principal: Ribeiro, Maycon Carvalho
• Orientador(a): Amaral, André Corrêa
• Banca de defesa: Amaral, André Corrêa, Vieira, José Daniel Gonçalves, Fernandes, Éverton Kort Kamp
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Goiás
• Programa de Pós-Graduação: Programa de Pós-graduação em Genetica e Biologia Molecular
• Departamento: Instituto de Ciências Biológicas - ICB (RG)
• País: Brasil
• Palavras-chave em Português: Ciclodextrina glicosiltransferase; Bioprospecção; Índice enzimático
• Palavras-chave em Inglês: Cyclodextrina Glycosyltransferase; Bioprospecting; Production enzymatic
• Área do conhecimento CNPq: GENETICA::GENETICA MOLECULAR E DE MICROORGANISMOS
• Link de acesso: http://repositorio.bc.ufg.br/tede/handle/tede/4730

Cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) is an important industrial enzyme for being the only one able to convert starch and related glucans in cyclic oligosaccharides called cyclodextrins (CDs). The arrangement of the glucose units in the formation of CD results in a molecule with the shape of a cone, with hydrophobic interior and hydrophilic surface. This arrangement of glucose molecules in CDs allows its use as a host molecule in the formation of inclusion complexes with organic and inorganic compounds. This mechanism is advantageous in protecting the guest molecule from light, heat and oxidizing conditions and also enable the "dissolution" of compounds of low solubility in aqueous media. Cyclodextrins are used from the food industry to the pharmaceutical, in controlled drug delivery systems and immobilization of toxic compounds for environmental protection. The CGTases are mainly produced by bacteria of the genus Bacillus, found degrading starch rich substrates. The aim of this study was to identify, isolate, select and characterize strains of CGTase-producing bacteria from soil samples from different regions of Brazil as well as calculate the enzymatic production of these bacteria on low-cost substrates. With this work, it was possible to identify 17 bacteria producing cyclodextrin glycosyltransferase enzyme, with nine of them had values above 1.5 for enzymatic production. Of these, all were characterized as gram positive Bacillus. Bioprospecting of bacteria in soils of different cultures led to the identification of bacteria that may be used in studies for the production of cyclodextrin glycosyltransferase and subsequent implementation by various industries.