Avaliação do uso de fatores de ressuscitação no sistema BACTEC MGIT 960 para detecção de Mycobacterium tuberculosis em amostras paucibacilares

• Ano de defesa: 2024
• Autor(a) principal: Araújo, Filipe Augusto Coimbra de
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Espírito Santo; BR; Mestrado em Doenças Infecciosas; Centro de Ciências da Saúde; UFES; Programa de Pós-Graduação em Doenças Infecciosas
• Palavras-chave em Português: Mycobacterium tuberculosis; Tuberculose; Dormência; Filtrado de cultura; BACTEC MGIT; Tuberculosis; Dormancy; Culture Filtrate; Doenças Infecciosas e Parasitárias
• Link de acesso: http://repositorio.ufes.br/handle/10/18179

The dormancy of Mycobacterium tuberculosis (Mtb) challenges laboratory diagnosis, as dormant subpopulations are not easily detected by conventional methods. The objective of this study was to evaluate the use of culture filtrate (CF) in the BACTEC MGIT 960 system to improve the detection of Mtb in 245 paucibacillary clinical samples from patients suspected of having tuberculosis (TB). The study compared samples inoculated in MGIT medium with and without the addition of Mtb CF. The results showed that the addition of CF increased the sensitivity of the test, particularly in extrapulmonary samples, with sensitivity and specificity for MGIT+CF being 97.50% and 97.86%, respectively. In comparison, the Xpert MTB/RIF Ultra presented a sensitivity of 60.53% and a specificity of 93.97%, while the egg-based medium showed 40% sensitivity and 91% specificity. Furthermore, the use of CF increased the frequency of contamination in liquid cultures and did not alter the time to positivity of MGIT. These findings suggest that the use of CF may be a promising tool for enhancing the accuracy of TB diagnosis in samples with low bacterial load