Genótipos de HPV em espécimes cervicais e anais de mulheres sem lesão cervical e soropositivas para HIV

• Ano de defesa: 2016
• Autor(a) principal: Volpini, Lays Paula Bondi
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Espírito Santo; BR; Mestrado em Doenças Infecciosas; Centro de Ciências da Saúde; UFES; Programa de Pós-Graduação em Doenças Infecciosas
• Palavras-chave em Português: Genotyping; Variants; HPV; Genotipagem; Variantes; Papillomavírus humano; HIV (Vírus); Doenças Infecciosas e Parasitárias; 61
• Link de acesso: http://repositorio.ufes.br/handle/10/4591

The human papillomavirus (HPV) infection is the main risk factor for the development of anogenital cancers. High-risk HPV (HR-HPV) genotypes and their variants are associated with the development of malignant lesions. Others risk factors, as HIV infection, increases the persistency of HPV infection and HPV-related cancer. Molecular methods for HPV detection and typing can be useful for triage of women with negative cytology, especially those HIV seropositive. This study aimed to characterize the HPV types and HPV16 variants in cervical and anal specimens of HIV seropositive women, with normal cervical cytology, attended at Reference Center for STD/AIDS, Vitória-ES. Viral DNA was screened by PCR with set of primers PGMY09/11 from nucleic acid extracted with QIAamp DNA Mini Kit™ (QIAGEN). The genotype was determined by Reverse Line Blot (RLB), Restriction Fragment Length Polymorphism (RFLP) and genetic sequencing, and HPV16 variants, by sequencing. HPV DNA was detected in a total of 71.4% (90/126) of women, 38.9% (49/126) in cervical and 60.3% (76/126) in anal samples; 38.9% (35/90), at both sites concomitantly. Thirty-four HPV different types have been identified, being the HR-HPV found in 83.7% (41/49) and 77.6% (59/76) of cervical and anal samples, respectively. HPV16 was the most prevalent type in both sites, followed by types 45> 31,35,44,69> 18,52,66 in the cervical region and by types 44> 6> 53 in the anal region. European variant corresponded to 70.8% (17/24) of HPV16 cases and non-European, to 29.2% (7/24). A same HPV genotype was present in both anatomical sites in 48.6% of the samples, 76.5% were HR-HPV. Infection with at least three HPV types was common, being in most cases, in the anal region (78.6%). Among the variables investigated, age of 18-35 years, CD4 <500 cells/mm3 and HIV viral load >50 copies/cel were statistically related to the presence of anal HPV. The high frequency of HR-HPV in women of a risk group with normal cervical cytology reinforce the discussion concerning the improvement of screening policies of cervical cancer for this population.