Efeitos cardiometabólicos da mobilização de gordura subcutânea induzida por ultra-som terapêutico: evidências de um modelo de síndrome metabólica em ratas

• Ano de defesa: 2008
• Autor(a) principal: Gonçalves, Washington Luiz Silva
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Espírito Santo; BR; Doutorado em Ciências Fisiológicas; Centro de Ciências da Saúde; UFES; Programa de Pós-Graduação em Ciências Fisiológicas
• Palavras-chave em Português: Ultrasound; White adipose tissue; Dyslipedemia; Energy metabolism; Coronary dysfunction; Metabolic syndrome mode; Ultra-som; Tecido adiposo branco; Dislipidemias; Resistência insulínica; Metabolismo energético; Disfunção coronariana; Modelo de síndrome metabólica; Fisiologia; 612
• Link de acesso: http://repositorio.ufes.br/handle/10/5163

Recent studies demonstrate that the ultrasound therapy increase adrenergic activity in the white adipose tissue (WAT), and produces local lipolysis with relevant fat corporal mobilization, currently effect described as ultrasound lipoclasia (USL). The aim of the present study was to investigate whether the peripheral USL can predispose to the development of metabolic syndrome components. Thirty female Wistar healthy rats (3 mo old) weighing ≈ 300g, were divided into sham-stimulated (SHAM, n=10), ultrasound-stimulated (LIUST, n=10), and washout-stimulated (WASHOUT) groups. An ultrasonic irradiation of 3 MHz was applied at the gluteusfemoral region in LIUST and WASHOUT groups. The spatial average and temporal average intensity ISATA was 5.6W/cm2 , pulsed mode 2ms: 8ms for 3 min during 10 days to produce local fat mobilization Anthropometrical, body composition, biochemical and hemodynamic parameters, and isolated heart studies were performed. All values are expressed as mean ± SEM. One- or two-way analysis of variance (ANOVA) followed by Tukey test was used for statistical analysis. The significance level was set at * p<0.05, **p<0.01, vs. group SHAM and # p<0.05, ##p<0.01, vs. LIUST group. In 10º day, the fat mass (38±1, 31±1** and 44±2*## %), mean arterial pressure (99±2, 121±2** and 100±1## mmHg), insulin (1.4±03, 3.3±0.05** and 3.1±0.2** µU/mL), triglyceride (25±3, 50±2** and 35±4*## mg/dL), and plasma glucose (141±13, 208±7** and 108±00# mg/dL) were verified in SHAM, LIUST and WASHOUT animals, respectively. The groups LIUST and WASHOUT also presented changes of the plasma inflammatory response and coronary dysfunction with impairment of adenosine-induced vasodilation (-14±2, -3±2** and -9±2*# 19 mmHg). These results show that not visceral only, but peripheral fat mobilization by USL leads to the development of the metabolic syndrome components with coronary dysfunction, being able to be a suitable model for studies in metabolic syndrome in adult females.