Uso tópico do alendronato de sódio associado ao bio-oss® no reparo de defeitos ósseos : estudo histomorfométrico em calvária de ratos

• Ano de defesa: 2015
• Autor(a) principal: Musso, Douglas Bertazo
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Espírito Santo; BR; Mestrado em Clinica Odontológica; Centro de Ciências da Saúde; UFES; Programa de Pós-Graduação em Clínica Odontológica
• Palavras-chave em Português: Alendronate; Transplantation heterologous; Skull; Rat; Transplante heterólogo; Alendronato; Calvária; Cavidade dentária - Preparo; Ossos; Rato; Crânio; Odontologia; 616.314
• Link de acesso: http://repositorio.ufes.br/handle/10/8199

Alendronate of sodium has shown the anabolic effect in bone healing when associated in the grafts from lyophilized bovine bone, but a form of administration and the concentration are still under investigation. Objective: This study evaluated the effect of topical of 0.5% ALN, used alone or in association with the lyophilized bovine bone (Bio-Oss®), on the percentage of bone formation and absorption of graft. Methodology: Two 5mm diameter cavities were made in the cranium of 18 Wistar rats and filled with 0.5% ALN, broadcasted in gelatin sponge (Group A – GA) Bio-Oss® (Group B – GB); Bio-Oss® soaked in 0.5% ALN (Group AB – GAB); blood clot (Control Group – GC). The animals were euthanized at 90 days postoperatively. The specimens were analyzed histologically (hematoxylin-eosin) and for histomorphometric analysis with the aid of AutoCAD® software, as the percentage of bone formation and remnants of Bio-Oss® granules. In the statistical analysis, the groups were compared using the “t” test of Student for paired samples (p<= 0.05). Results: The mean bone formation percentages were: GA = 40.4 ± 22.2%; GB = 46.0 ± 22.0%; GAB = 29.9 ± 17.8%; GC = 49.9 ± 16.4%, as long as the average of the percentages of the remaining granules of Bio-Oss was GB = 9.2± 4.5; GAB = 12.2 ± 7.2%. There was a significant statistical difference in the percentage of bone formation only between CG and GAB (p = 0.025), with the defect filled in without statistical difference as result of the presence of the granules of the graft. There was no significant statistical difference in the percentage of remaining granules between the groups. Conclusion: The topical use of ALN 0.5% isolated in the bone cavities did not change the percentage of bone formation. When combined with Bio-Oss®, decreased the percentage of bone formation and did not interfere in the reabsorption of the graft.