Aproveitamento de resíduos para produção de lipases fúngicas via fermentação em estado sólido

Detalhes bibliográficos
Ano de defesa: 2018
Autor(a) principal: Teodoro, Arthur Fagundes dos Santos
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal do Espírito Santo
BR
Mestrado Profissional em Engenharia e Desenvolvimento Sustentável
Centro Tecnológico
UFES
Programa de Pós-Graduação em Engenharia e Desenvolvimento Sustentável
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
628
Link de acesso: http://repositorio.ufes.br/handle/10/10899
Resumo: Lipases are enzymes responsible for the catalysis of reactions from a wide variety of industrial processes. Solid fermentation appears as an alternative for the production of enzymes due to the low cost and possibility of using residues of low value and with great abundance, besides requiring a smaller amount of energy in comparison to the submerged fermentation. This work had as objective to produce lipases by the fungi Penicillim sp. and Rhizomucor sp. by solid fermentation using sugar cane bagasse and coconut fiber. Initially, preliminary tests were carried out to test the use of a nutrient solution. After the tests, the enzyme production was optimized through an experimental design 33, where the influence of the inducer concentration (soybean oil) was studied in 2.5%, 5% and 10%, at a temperature of 27 ° C, 30 ° C and 33 ° C, and 40%, 60% and 80% field retention capacity for each medium and fungus. With the use of sugarcane bagasse as a support medium, Penicillium sp presented the highest lipase activity (147,5 U/g) at 30°C temperature, 60% field capacity and 5% of the inductor, with the Inductor Concentration and Temperature with significant influence on the results. Rhizomucor sp had the highest lipase activity (161,3 U/g) under the same conditions mentioned above and with the same significant influence on the results. For coconut fiber, Penicillium sp presented the highest lipase activity (75 U/g) under conditions of 30 ° C temperature, 60% humidity and 10% of the inductor, with field capacity, concentration of the inductor and temperature with significant influence on results. Rhizomucor sp had the highest lipase activity (78,9 U/g) under the same conditions mentioned above and with the same influence in the results.