Estudo químico de Pavonia multiflora A. St-Hil. (Malvaceae), planta endêmica do Espírito Santo

• Ano de defesa: 2014
• Autor(a) principal: Lopes, Leandra Gobira
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Espírito Santo; BR; Mestrado em Química; Centro de Ciências Exatas; UFES; Programa de Pós-Graduação em Química
• Palavras-chave em Português: Phytochemistry; Natural products; Produtos naturais; Química vegetal; Pavonia multiflora; Catepsinas; Química; 54
• Link de acesso: http://repositorio.ufes.br/handle/10/1613

This paper describes the chemical study of Pavonia multiflora leaves. The study targeted the isolation and identification of the species’ secondary metabolites, which can be used to search for biological activity. P. multiflora is endemic of the state of Espírito Santo and is found in the Atlantic Forest region. This species has not yet been recorded in phytopharmacologics studies in the scientific community. Therefore, in this work, the ethanolic extract, from the leaves of the P. multiflora, was partitioned to the partitions of hexane and of ethyl acetate. Such partitions were subjected to various chromatographic methodologies, including a thin layer chromatography , an in column liquid chromatography, preparative thin layer chromatography, vacuum liquid chromatography, and a high-performance liquid chromatography using diverse stationary phases and eluents, to isolate the chemical components. Using spectroscopic and spectrometric techniques, ten substances, including four phenolic compounds (p-hydroxybenzoic acid, p-coumaric acid, vanillic acid and ferulic acid), five terpene derivatives (loliolide, vomifoliol, 4,5 dihydroblumenol A, 3-oxo-a-ionol and blumenol C), and an esterified derivative of triterpenoid taraxerol, taraxerol p-methoxybenzoate (novel compound), not previously described in this species. The total leaf extract was tested at different concentrations to determine if it is an inhibitor of cathepsins K, L and V, and these tests showed cathepsin K and V inhibitory properties at a concentration of 500 µg/mL.