Avaliação dos efeitos do fator estimulador de colônias de granulócitos na isquemia/reperfusão renal

• Ano de defesa: 2014
• Autor(a) principal: Rodrigues, Vinicius Correa
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal do Espírito Santo; BR; Mestrado em Biotecnologia; Centro de Ciências da Saúde; UFES; Programa de Pós-Graduação em Biotecnologia
• Palavras-chave em Português: Ischemia; Reperfusion; Renal function; Collagen; Função renal; G-CSF; Isquemia; Rins; Colágeno; Reperfusão (Fisiologia); Biotecnologia; Fator etimulador de colônias de granulócitos; 61
• Link de acesso: http://repositorio.ufes.br/handle/10/1895

Ischemia/reperfusion (IR) in kidney obtained by temporary occlusion of the renal artery causes injuries similar to cases of acute renal failure founded in clinical practice. Several therapeutic strategies have been studied to minimize the consequences of the damage caused by IR. The aim of this study was to evaluate the effects of granulocyte colony-stimulating factor (G-CSF) in an animal model of renal ischemia and reperfusion after the ischemic event. In the present study male Wistar rats weighing between 150-250 g were used. We performed a dose-response curve to establish a safe dose of G-CSF without loss of kidney function at concentrations of 10, 50, 100 and 250 mg/kg/day for 5 days. For the study of IR animals were distributed in three groups: SHAM (n=10) (control, without clamping, with unilateral nephrectomy) who received reperfusion 5% glucose solution (vehicle), IR (n= 9) (IR unilateral nephrectomy) and IR- GCSF (n = 9) (IR injury associated with G-CSF 100 mg/kg for 3 days). Ischemia was achieved by clamping the renal artery for 40 minutes followed by reperfusion. The animals were placed in metabolic cages for analysis of parameters of renal function and subsequently euthanized with an overdose of anesthetic for blood and organs of interest. The results were normalized and the ANOVA-1 followed via a post hoc Tukey was used, the values are presented as mean ± SEM and considered significant when p<0.05. At the end of the treatment the amount of leukocytes of the animals was 10370 cells/dL (sham), 11775 cells/dL (IR) and 28800 cells/dL (IR- GSCF) (p < 0.05, vs. sham IR and IR-GCSF). The plasma creatinine values were 0.34 ± 0.02 , 0.43 ± 0.03 and 0.33 ± 0.02 mg / dL for animals in groups SHAM , IR and IR- GSCF , respectively (p<0.05, vs IR IR-GCSF). The percentage area of collagen deposition in the medullary area was 16.22 ± 3.41 %, 32.99% ± 1.17 and 20.0 ± 1.20 % for the SHAM, IR and IR- GSCF groups respectively (p < 0.01, IR vs IR-GCSF). There was even smaller amount of cells undergoing apoptosis and lower concentration of advanced oxidation protein products in treated animals when compared with the IR group. These results demonstrate the protective effect of G-CSF against renal injury in an experimental model of IR.