Estudo químico, toxicológico e efeito neuroprotetor do maracujá alho (Passiflora tenuifila Killip)

• Ano de defesa: 2019
• Autor(a) principal: Holanda, Dayse Karine Rodrigues
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Palavras-chave em Português: Componentes funcionais; Antioxidante; Ansiolitico; Toxicidade; Doença de Parkinson
• Link de acesso: http://www.repositorio.ufc.br/handle/riufc/47592

Several species of the genus Passiflora are its sedative and soothing properties. Passiflora tenuifila is a type of non-commercial passion fruit known as garlic passion fruit and is among the least known, with few scientific studies in the literature with their different properties. The aim was to characterize the ingredients present in passion fruit and to evaluate the toxicity and effects on the Central Nervous System (CNS) in the animal models. The fruits were ground whole and lyophilized. After, were characterized in the physical-chemical and phytochemical analytics. Toxicity was evaluated in in vitro and in vivo models. Anti-inflammatory activity was evaluated in vitro with human neutrophils. Anxiolytic, antidepressive, sedative and anticonvulsant effects were evaluated in a behavioral model using Swiss mice and the evaluation of the antiparkinsonian effect was performed with Wistar rats using rotenone as an inducing agent for Parkinson's symptoms. Garlic passion fruit presented a moisture content of 4.99%, 6.29% of ash, 8.77% of proteins, 10.93% of lipids and 69.19% of carbohydrates, of these 48.40% correspond to the content of total dietary fiber. Regarding the total polyphenol content and total antioxidant activity, 1151.34 mg EAG.100 g-1 and 109.71 μM Trolox.g-1, respectively. In the chemical composition by Nuclear Magnetic Resonance (NMR) were identified sugars, organic acids and amino acids and by Ultra-High Performance Liquid Chromatography (UPLC), phenolic compounds, mainly phenolic acids and flavonoids. There was no in vitro toxicity and in vivo tests showed that the administrations of garlic passion fruit used did not cause any signs of toxicity in relation to the evaluated parameters. Anti-inflammatory activity was no observed in human neutrophils at the doses used. In the functional effect, the animals treated with garlic passion fruit (200 and 400 mg.kg-1) showed a decrease in the locomotor activity in the open field test and without alterations in the motor coordination in the test of the routed rod, indicating a sedative activity without muscle relaxation. In addition, they also showed an increase in the behavior of head dips in the hole board test, indicating an anxiolytic activity. The forced swimming test was performed to assess antidepressant activity, but no significant effect was observed at the doses used. Garlic passion fruit (200 and 400 mg.kg-1) potentiated sleep induced by ethyl ether, showing a hypnotic-sedative activity. In the seizure test induced by pentylenetetrazol, it was observed that the garlic passion fruit (400 mg.kg-1) exerted a protective effect, reducing the severity of the seizures and not causing the death of the animals. In the anti-parkinsonian effect, behavioral tests indicated that consumption of garlic passion fruit caused an improvement and possible recovery effect of rotenone-induced damage in the CNS, and in addition, caused an increase in the level of dopamine in the brains of animals in the dose of 400 mg.kg-1. It can be concluded that garlic passion fruit presented no toxicity, being safe for human consumption, and presented promising functional effect as anxiolytic agent, hypnotic-sedative, anticonvulsive and antiparkinsonian, possibly related to the phenolic compounds present.