Cárie oculta: revisão de literatura, relato de caso e perfil microbiano

• Ano de defesa: 2023
• Autor(a) principal: Vieira, Pedro Henrique Acioly Guedes Peixoto
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Palavras-chave em Português: Cárie Dentária; Dentina; RNA Ribossômico 16S; Microbiota
• Link de acesso: http://www.repositorio.ufc.br/handle/riufc/71342

Hidden caries (HC) is a type of dentin lesion that is not easily identified with silent evolution and its progression can compromise the dentin-pulp complex and, in more severe cases, lead to tooth loss. This thesis, distributed in three chapters, had the following objectives: to carry out a literature review on the current challenges in occult caries lesions (Chapter 1), as well as to describe an approach and form of restorative treatment based on a case report ( Chapter 2) and identify the microbial composition, through RNA sequencing, in Exception of carious dentin from occult carious lesions in permanent teeth (Chapter 3). For a review of related factors, prevalence, microbiota, diagnostic and management methods for occult caries lesions were considered. There is a high prevalence in the young adult population, where its diagnosis requires sensitivity and specificity and a still little known etiology. The activity, extension and location of the lesion are criteria evaluated for a better therapeutic approach to HC lesions. and without painful symptoms. After six months, the patient was pain-free, with healthy periapical and periodontal parameters, as well as reinforced restorative treatment. The occlusal replication technique showed an efficient alternative, optimizing the reconstruction of the original occlusal morphology of tooth 27. In the molecular identification of microorganisms present in 18 samples of HC lesions, the presence of the genera Olsenella, Lactobacillus and Prevotella was observed in most sequenced samples. However, Olsenella spp. showed about 20-50% in 7 samples of HC lesions. In addition, they were found at the species level such: Stomatobaculum_longum (Med. 0.88, p=0.03), Prevotella_oris (Med. 0.52, p =0.03), Lachnoanaerobaculum_NA (Med. 0.05, p=0.03) Shuttleworthia_satelles (Med. 1.42, p=0.03) and Propionibacterium_acidifaciens (Med. 0.70, p=0.04) in deeper occult carious lesions. Thus, it is suggested that the analyzed microorganisms, at the genus and species level, may be strongly associated with HC lesions in the treated population.