Géis de colágeno e gelatina do resíduo da tilápia (Oreochromis niloticus) para incorporação e liberação controlada in vitro da astaxantina

• Ano de defesa: 2017
• Autor(a) principal: Oliveira, Jakson Martins de
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Palavras-chave em Português: Tilápia; Hidrogéis; Astaxantina; Colágeno; Gelatina
• Link de acesso: http://www.repositorio.ufc.br/handle/riufc/31601

The use of gels as drug carrier is a promising proposal because it aims to controlled release of an active ingredient in a specific location of the body for a longer period of time than conventional administration, keeping a therapeutic concentration and avoiding the need for repetition of the Administration. Using the data cited as reference this work aims formulate and characterize collagen and gelatin hydrogels lattices with caffeic acid for incorporation and controlled release of astaxanthin. Collagen and gelatin were extracted from the skin of Tilapia by acid solubilization method 4 ºc and extraction method in water at 60 °C, respectively. Characterization was carried out the analysis of MEV, TGA, DSC, DRX, FTIR, gel, pH and chemical composition. Collagen and gelatin were used for the preparation of hydrogels in order to evaluate the influence of caffeic acid (crosslinking agent) about attributes of collagen and gelatin gels. The hydrogels incorporated with astaxanthin has been prepared homogenizing in Ultra Turrax gelatine or collagen to SPAM 80, sunflower oil and potassium sorbate, after it was incorporated into astaxanthin and finally the caffeic acid. The hydrogels incorporated with astaxanthin were characterized as the kinetic profile of release. The FTIR spectra of gelatine and collagen in ratified efficiency in extraction. Gelatine had gel strength of 438 g and collagen 68 g. The FTIR spectra confirmed that caffeic acid was effective in crosslinking of hydrogels. The thermograms of hydrogels of gelatine and collagen showed an increase in thermal stability commensurate with the levels of caffeic acid in gels. The results of crystallinity indices were lower in hydrogels lattices. The texture of the crosslinked gelatin hydrogels were higher in detriment to those without crosslinking. GAs0,02Ac1 and CAs0,02Ac1 treatments were responsible for the longer release kinetics. Gelatin hydrogels had a rapid release of astaxanthin in about 15 min, whereas those with collagen were found to be longer lasting for a maximum time of 5 hr 5 min at 6 h 30 min of experiment. The release content of the non-crosslinked hydrogels for collagen and gelatin was 91%, with the lowest value identified among the treatments.