Extração e separação de flavonóides do extrato da casca do maracujá

• Ano de defesa: 2018
• Autor(a) principal: Pinto, Paloma Lira
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Palavras-chave em Português: Engenharia química; Maracujazeiro-amarelo; Maracujá - Subprodutos; Passiflora; Flavonóides; Biphasic aqueous system; By-products; Passiflora edulis f. flavicarpa
• Link de acesso: http://www.repositorio.ufc.br/handle/riufc/31746

Passion fruit peel is a by-product of the flavonoid-rich food industry that has several health benefits, and can be extracted using several methodologies, including the aqueous two-phase system (ATPS). It is an efficient liquid-liquid extraction technique that confers high yields while preserving the biological activity of the compounds. This work aimed to separate flavonoids from passion fruit peel (previously extracted through pressurized solvent extraction) using ATPS consisting of ethanol, salt and water. The influence of the two types of salts, ammonium sulfate and potassium phosphate, and the concentration of ATPS components on the partition and recovery of flavonoids were evaluated. Initially, ethanol concentration was varied by setting the salt concentration. The ethanol variation was from 26.5 to 32.19%, with the salt being fixed in 18.5% in Ethanol/(NH4)2SO4 system, and 19.9 to 25.3%, with the salt being fixed 22.03% in Ethanol/K2HPO4 system. Thereafter, the salt concentration was varied by setting the ethanol concentration. The salt variation was from 14.86 to 18.5%, with the ethanol being set at 30.14% in ethanol/(NH4)2SO4 system, and 18.10 to 26.09%, with the ethanol being set at 21.26% in Ethanol/K2HPO4 system. The analytical determinations were: yield, pH, total polyphenol content, total sugars and liquid chromatography coupled to mass spectrometry. Partition coefficient and recovery were calculated. In both systems, the partition of flavonoids was higher than 70, and that of sugars varied between 0.01 and 0.03. The recovery of flavonoids and sugars was above 99% and 92% in the Ethanol/(NH4)2SO4 system, and above 98% and 96% in the Ethanol/K2HPO4 system, respectively. The elevation of ethanol concentration did not significantly influence the recovery of the compounds. On the other hand, the increase in the concentration of salts caused an increase in the recovery of sugars from 92.98% to 97.25% in the Ethanol/(NH4)2SO4 system and from 96.7% to 98.73% in the Ethanol/K2HPO4 system. ATPS was shown to be effective for the separation of flavonoids. Both salts were suitable for the separation, paying attention to the pH conferred to the system. The passionfruit peel was source of 12 different types of flavonoids, being a good and low cost alternative to obtain these bioactive compounds.