Detalhes bibliográficos
Ano de defesa: |
2011 |
Autor(a) principal: |
Carvalho, Eunice Bobô de |
Orientador(a): |
Não Informado pela instituição |
Banca de defesa: |
Não Informado pela instituição |
Tipo de documento: |
Tese
|
Tipo de acesso: |
Acesso aberto |
Idioma: |
por |
Instituição de defesa: |
Não Informado pela instituição
|
Programa de Pós-Graduação: |
Não Informado pela instituição
|
Departamento: |
Não Informado pela instituição
|
País: |
Não Informado pela instituição
|
Palavras-chave em Português: |
|
Link de acesso: |
http://www.repositorio.ufc.br/handle/riufc/7272
|
Resumo: |
The enteric infections cause 2.5 million deaths each year. The Enteroaggregative Escherichia coli (EAEC) is associated with persistent cause of diarrheal diseases. This study examined in vitro (IEC-6, Caco-2 and HEp-2 cells) the role of the micronutrients glutamine (Glu), alanyl-glutamine (Ala-Glu), beta-carotene (ß-Carot), zinc (Zn), and the milk of transgenic goats containning human lysozyme (M-Lyso) and their respective controls (Ctrle) in the following assays: proliferation, migration, viability, apoptosis, cell necrosis, and bacterial adhesion in response to infection caused by the EAEC-042 bacterial strain at a concentration of 2.5 x 105 CFU/mL. The effect of infection by EAEC-042 bacterial strain was evidenced by significant reduction in migration (p <0.001) and cellular viability (p <0.001); also increased apoptosis (p <0.001) and necrosis (p <0.001) in response to damage to the intestinal epithelium. It was observed that the micronutrients in the presence of bacteria significantly reduced apoptosis and necrosis caused by EAEC-042, as well as significantly reduced bacterial adhesion and increases cell migration. The control and transgenic milk abolished bacterial adhesion (p <0.001), independent of milk fat, and significantly reduce apoptosis (p <0.001) and necrosis (p <0.001) caused by EAEC-042. The qualitative analysis of EAEC adherence, considered as gold standard method, showed a reduction in bacterial adherence associated with intervention with micronutrients when compared with the EAEC-042 infection control. In conclusion, our study demonstrates the importance of intervention with micronutrients and milk (transgenic or not) in protecting the intestinal epithelial challenged by bacterial aggression. |