A própolis vermelha e l-lisina em lesões pré-neoplásicas colorretais induzidas pelo azoximetano

• Ano de defesa: 2018
• Autor(a) principal: Braga, Vanessa Nogueira Lages
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Palavras-chave em Português: Neoplasias Colorretais; Própole; Lisina; Biomarcadores Tumorais
• Link de acesso: http://www.repositorio.ufc.br/handle/riufc/32698

Colorectal cancer (CRC) is a third most common malignant neoplasm in the world. Dysplastic aberrant crypt outbreaks (ACF) are important markers for colorectal cancer. Propolis is a resinous product, used to protect a hive, and has antitumor properties. L-Lysine is an essential amino acid reported as promoter of chemical carcinogens in the rat bladder. The objective of this work was to analyze effects of red propolis and L-lysine on azoxymethane-induced colonic pre-neoplastic lesions (AOM - 15mg / kg intraperitoneal). Fourty-eight wistar rats were divided into eight groups denominated according to the substance administered along 16 weeks: GI-water, GII-L-lysine, GIII-red propolis, GIV-arabic gum 1%, GV-AOM + water, GVI-AOM + L-lysine, GVII-AOM + red propolis and GVIII-AOM + 1% gum arabic. Dysplastic aberrant crypt were quantified and analyzed oxidative stress (Glutathione and TBARS), genotoxicity through comet assay in peripheral blood and micronucleus in peripheral blood and bone marrow, as well as registred the reticulocyte / erythrocyte ratio in peripheral blood. Red propolis reduced the total number of ACF in the distal colon in animals receiving AOM and propolis (p <0.01), and in every colonic segment considering the number of ACF up to 4 crypts (p <0.05). L-lysine does not demonstrate protective or promoter effect on pre-neoplastic lesions induced by AOM. Red propolis and L-lysine have not demonstrated protective effects in genotoxicity or mutagenesis in groups that are submitted also to AOM at the doses and times administered. Oxidative stress (TBARS) was shown in all animals receiving AOM, and red propolis reduced oxidative stress (p <0.01). Gum arabic, used to extract própolis, showed a protective action reducing the distal colon ACFs (p <0.05) considering the total number of ACFs, as well as throughout the colonic segment reducing the number of FCAs with up to 4 crypts (p <0.05), in those ones receiving AOM and gum. Therefore, the results indicated that red propolis and gum arabic had protective action reducing pre-neoplastic lesions in colorectal carcinogenesis induced by AOM. Red propolis also reduced the oxidative stress caused by the carcinogen.