Estudo da atividade antinociceptiva e antiinflamatória da riparina II (O-metil-N-2-hidroxi-benzoil tiramina) em modelos experimentais

• Ano de defesa: 2011
• Autor(a) principal: Carvalho, Alyne Mara Rodrigues de
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Palavras-chave em Português: Nociceptividade; Inflamação
• Link de acesso: http://www.repositorio.ufc.br/handle/riufc/2192

Riparin II, an alcamide isolated from the green fruit of Aniba riparia, was tested in animal models, such as acetic acid-induced abdominal writhing, formalin test, hot plate test, capsaicin and glutamate-induced nociception, paw edema induced by carrageenan, dextran, histamine and serotonin and peritonitis induced by carrageenan and fMLP, in order to evaluate its antinociceptive and antiinflamatory activities. Male Swiss mice (20-30g) and male Wistar rats (150-250g) were treated by gavage with riparin II at the doses of 25 and 50 mg/kg. The results show that riparin II demonstrated an antiinflammatory activity at the animal model of acetic acid-induced abdominal writhing. The pretreatment with riparin II reduced significantly the nociception induced by glutamate and capsaicin, and also the inflammatory nociception induced at the second phase of the formalin test, but was not able to reduce the neurogenic nociception induced at the first phase of the test. Riparin II did not show any activity at the hot plate test. At the animal models of paw edema induced by carrageenan and dextran, the animals treated with riparin II showed lower edema when compared to the control group. The treatment with Riparin II was able to reduce the paw edemas induced by histamine and bradykinin, but not the edema induced by serotonin. At the model of peritonitis induced by carrageenan (indirect chemotactic agent) and by fMLP (direct chemotactic agent), the treatment with riparin II reduced the influx of leukocytes, the activity of the enzyme myeloperoxidase and also the levels of TNF-α and IL-1β in the peritoneal fluid. In summary, these results indicate that riparin II has an antinociceptive and antiinflammatory activity in chemical models of nociception and inflammation. Besides that, riparin II might be inhibiting directly or indirectly the activity, production or releasing of pro-inflammatory mediators involved at the generation of pain and inflammation. The antiinflammatory activity was confirmed through the histopathological findings, with the reduction of the edema and the inflammatory infiltrate.