Alterações comportamentais e envolvimento do estresse oxidativo provocadas por diferentes tratamentos com ketamina em camundongos

• Ano de defesa: 2012
• Autor(a) principal: Silva, Francisca Charliane Carlos da
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Palavras-chave em Português: Ansiedade; Depressão; Ketamina
• Link de acesso: http://www.repositorio.ufc.br/handle/riufc/7230

Ketamine is mainly used as an anesthetic, producing a kind of anesthesia, called dissociative anesthesia characterized by a cataleptic. However, the use of ketamine has been restricted to clinical practice and research, since, currently, has been used as a drug of abuse, since both produce hallucinogenic effects as stimulants. The aim of this study was to evaluate behavioral changes such as depression and anxiety in mice subjected to different treatments with ketamine (acute, subchronic and withdrawal of 24), as well as investigate the involvement of oxidative stress in the prefrontal cortex of mice after acute treatment Ketamine. This is an experimental study to evaluate where behavioral changes (depression and anxiety) mice were treated with ketamine acutely or subcronicamente (7 consecutive days), being conducted experimental models of Open Field (TCA), Labyrinth of High Cross (LCE), Forced Swim (TNF) and Tail Suspension (TSC). The animals also were exposed to 24 hours of abstinence after treatment and subjected to subchronic experimental models above. To investigate the involvement of oxidative stress, the animals were treated acutely with ketamine and used the prefrontal cortex to evaluate the activity of catalase, glutathione, and nitrite malonialdeído. The results showed that the TCA increased spontaneous locomotor activity in all types of treatment and at all doses used. In LCE treating acute and subchronic withdrawal 24h with ketamine reduced some parameters in the open arms. In TNF and TSC, acute and subchronic treatment reduced immobility time at all doses used. While the withdrawal of 24h was increased immobility time in doses of 10 and 20 mg/kg. Regarding the involvement of oxidative stress after acute treatment with ketamine in the prefrontal cortex of mice was noted a significant increase in catalase activity, levels of malondialdehyde and nitrite. While the levels of reduced glutathione (GSH) showed a significant decrease. It concludes with the work that the acute and subchronic treatment caused anxiogenic and antidepressant effects. While abstinence from 24h after treatment showed a subchronic effect anxiogenic and depressant. In addition to behavioral changes, it appears that acute treatment with Ketamine causes increased oxidative stress, indicating the possible occurrence of neural injuries as a result of these changes.