Potencial terapêutico do ácido cafeico no tratamento da obesidade e cultivo celular de pré-adipócitos 3T3-L1

• Ano de defesa: 2019
• Autor(a) principal: Branco, Mariana Brito Dantas Castelo
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Palavras-chave em Português: Obesidade; Adipócitos; Ácidos Cafeicos
• Link de acesso: http://www.repositorio.ufc.br/handle/riufc/51035

Obesity is a chronic disease of multifactorial cause, whose incidence and persistence in adults is associated with the development of chronic diseases and increased risk of early mortality. In this context, Brazilian biodiversity has great potential for the discovery of molecules that have therapeutic potential. Among the substances obtained from vegetables, there is caffeic acid (CA) that can be useful in the treatment of various pathologies, such as obesity. The objective of this work is to evaluate the effect of CA in experimental protocols of hypercaloric diet-induced obesity, as well as to verify its activity in adipocytes. Male swiss mice were divided into groups (n = 8): standard diet (SD), hypercaloric diet (DH), sibutramine 10mg/kg (SIB), caffeic acid 12.5mg/kg (AC 12.5), caffeic acid 25mg/kg (AC 25) and caffeic acid 50mg/kg (AC 50). Obesity was induced by eating a high calorie diet prepared at the Pharmacotechnique Laboratory of the Pharmacy Course/UFC. Concomitant treatment protocols to obesity induction and posttreatment with AC were performed. The animals received standard diet (SD) or hypercaloric diet (DH, SIB, AC12.5; AC25 and AC50) and were treated according to group and protocol. During the experimental protocols, the weight and water and feed intake of the animals were measured, as well as the determination of glucose, total cholesterol, triglycerides, AST, ALT, insulin and adiponectin. A model of pre-adipocyte differentiation into adipocytes with simultaneous treatment to the process of differentiation and post-treatment with CA was also performed. Results were expressed as mean ± S.E.M. and the groups were compared by ANOVA (Turkeyi post-test), adopting as significance criterion p <0.05. The protocol described was approved by the UFC Animal Research Ethics Committee under No. 61/2013. In experimental models of obesity-induced obesity by hypercaloric diet there was a greater than 20% increase in the weight of the DH group in relation to PD. Animals treated with caffeic acid simultaneously with obesity induction showed a significant reduction of their weight (p <0.05) at 25 (AC 25) (40.89 ± 0.99 g) and 50 mg/kg doses. (AC 50) (40.08 ± 0.74 g), by 10.13% and 11.91%, respectively, when compared to the DH group (45.50 ± 1.40 g). Simultaneous treatment with caffeic acid also reduced the glycemia and total cholesterol of animals in groups AC 25 and AC 50. While post-treatment only the highest dose (50 mg/kg) was able to significantly reduce animal weight. by 11.4%. In in vitro assays, caffeic acid's ability to reduce lipid accumulation related to differentiation of 3T3-L1 pre-adipocytes into adipocytes was verified. After DCF-DA assay, caffeic acid was found to reduce the production of reactive oxygen species in the model and a significant increase in cell mitochondriabound rhodamine was also shown in both models used and at the two concentrations analyzed during differentiation. of the cells. The results of this study suggest that caffeic acid has potential anti-obesity.