Avaliação do perfil oxidativo e da genotoxicidade em pacientes submetidos a transplante de células tronco hematopoéticas autólogo

• Ano de defesa: 2014
• Autor(a) principal: Santos, Thayna Nogueira dos
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Palavras-chave em Português: Transplante de Medula Óssea; Estresse Oxidativo; Genotoxicidade
• Link de acesso: http://www.repositorio.ufc.br/handle/riufc/13594

Hematopoietic Stem Cell Transplantation (HSCT) is an autologous therapeutic method in which the stem cells are collected from th e individual’s own peripheral blood to restore spinal cord function. This is a prospective longitudinal study aimed to investigate the genotoxicity and oxidative profile in adult patients with multiple myeloma, Hodgkin’s lymphoma and non - Hodgkin's lymphoma undergoing autologous HSCT and in apparently healthy individuals, from January 2013 to December 2013. Samples of heparinized and EDTA blood of 37 patients befor e the conditioning regimen (CR) , were collected 24 hours after CR (D - 1) , 1 day (D +1) , 10 da ys (D +10) and 20 (D +20) days after HSCT and from 30 apparently healthy individuals. Oxidative stress parameters were analyzed: MDA (malondialdehyde), nitric oxide (NO), catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD) as well a s genotoxicity, given by the Damage Index (DI) to DNA. MDA concentrations were significantly elevated in all groups of patients when compared to the control group at all stages of HSCT. There was statistical difference in nitric oxide concentration s betwee n patients and controls and among patients. The concentration of catalase was significantly reduced at D - 1 when compared to the pre - CR in the groups of patients, having recovered at D +20 in patients with MM and LH and at D+10 in patients with LNH in relat ion to D - 1. The concentration of glutathione peroxidase did not differ significantly in the groups of patients in the control group and between both groups of patients. The concentration of SOD was s ignificantly reduced in the pre - CR in MM patients compare d to the control group. There was a significant reduction for all patients at D - 1. The rate of DNA damage was statistically higher (p<0.05) in the pre - CR in the patient groups c ompared to the control group. D - 1 significantly increased compared to all other times and when analyzed in relation to the control group. Markers of oxidative stress and genotoxicity can be used as early biomarkers of toxicity assisting in the monitoring of patient progression and prognosis.