Avaliação da eficácia antimicrobiana do extrato hidroalcoólico dos frutos de punica granatum l. (romã) na placa bacteriana

• Ano de defesa: 2004
• Autor(a) principal: Menezes, Silvana Magalhães Siqueira
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Palavras-chave em Português: Microbiologia; Placa Dentária; Ortodontia
• Link de acesso: http://www.repositorio.ufc.br/handle/riufc/2633

The in vivo, evaluation, of the effect of the hydroalcoholic extract from Punica granatum L. (HAE) in the inhibition of the supragengival dental plaque, was carried with 60 voluntaries using fixed orthodontic appliance. The material was collected in the morning after 24h without oral hygiene, from 23 men and 37 women (age ranging from 9 to 25 years) at the city of Crato (Ceará), Brasil. The voluntaries were randomly distributed in to 3 groups of 20 subjects. Group 1: HAE; group 2: 0.12% Chlorhexidine gluconate (CLX); group 3 (control): distilled water. Each group received, under the form of a mouth-rinse for 1 minute, 15 ml of either HAE; CLX or distilled water. The dental plaque was collected before and after the mouth-rinse with one of these three substances. The bacteria colonies were counted and results expressed as colony forming unities (CFU). The minimum inhibitory concentration (MIC) and duration of the antimicrobial effect of HAE on the dental plaque were determined. The bacterial susceptibility tests in vitro to HAE and chlorhexidine on 14 pathogenic bacterial strains of the FMJ Bank, were assessed and MIC values for each bacterial strain susceptible to HAE were also determined. The statistics analysis (Mann-Whitney U) showed no difference between HAE and CLX groups (p = 0, 7251), but a significant difference was observed in the HAE group (p<0.0001) as compared against control. The percentages of inhibitions of the order of 83.5% and 79% after mounth-rinse with the HAE and CLX respectively. The MIC of the HAE on the microorganisms of the dental plaque was of 15 mg/ml. The antibacterial effect of the HAE lasted for at least 1h and the percentage of inhibition of the CFU was 72.2% after the mounth-rinse and 63.8%, 1h later. The HAE presents an antimicrobial efficacy similar to that shown by CLX, both being actives against S. aureus, S. β-hemoliticus, S. β-hemoliticus, P. aeruginosa, K. pneumoniae, P. vulgaris and E. coli. The HAE was also effective against C. albicans. The MICs of HAE on the strains of S. β-hemoliticus, S. aureus, P. aeruginosa and C. albicans were 1.87, 3.75, 7.5 and 15 mg/ml, respectively. The HAE presents a significant activity against microorganisms present in the dental plaque.