MORFOANATOMIA FOLIAR E CAULINAR, ANÁLISE QUÍMICA E ATIVIDADES BIOLÓGICAS DO ÓLEO VOLÁTIL DE Lavandula dentata L., LAMIACEAE

• Ano de defesa: 2016
• Autor(a) principal: Justus, Barbara
• Orientador(a): Farago, Paulo Vitor
• Banca de defesa: Budel, Jane Manfron , Kanunfre, Carla Cristine , Mello, João Carlos Palozzo de
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: UNIVERSIDADE ESTADUAL DE PONTA GROSSA
• Programa de Pós-Graduação: Programa de Pós Graduação Ciências Farmacêuticas
• Departamento: Farmacos, Medicamentos e Biociências Aplicadas à Farmácia
• País: BR
• Palavras-chave em Português: 1-8 cineol; antimicrobiano; antioxidante; citotoxicidade; morfoanatomia
• Palavras-chave em Inglês: 1-8 cineole; antimicrobial; antioxidant; citotoxicity; morphoanatomy
• Área do conhecimento CNPq: CNPQ::CIENCIAS DA SAUDE::FARMACIA
• Link de acesso: http://tede2.uepg.br/jspui/handle/prefix/99

Lavandula dentata L. presents as a small shrub, is native of the Mediterranean, with great pharmacological potential. The objective of this paper was study the morphoanatomy of the leaf, determine its chemical composition, antioxidant activity, antimicrobial activity and evaluate the cytotoxic potential of the volatile oil of L. dentata. Botanical material was collected in the Medicinal Garden of Pharmacy course at the State University of Ponta Grossa (Paraná). Diacytic stomata images obtained by optical microscopy and scanning electron microscopy, were evidenced only on the abaxial surface of the leaf blade, non-glandular branched trichomes, glandular capitate and peltate, dorsiventral mesophyll and concave-convex truncated midrib on the abaxial surface. The yield of volatile oil obtained by hydrodestilation of the aerial parts was 0.85%, and showed 1-8 cineole as main component identified by GC/MS. Volatile oil of L. dentata showed an antioxidant activity similar to rutin and gallic acid when analyzed by phosphomolibdenium method. However, by the free radical DPPH and ABTS method, it showed a slight potential antioxidant. The volatile oil showed antimicrobial activity against gram-positive, gram-negative bacteria strains and Candida albicans, by broth microdilution. This study, for the first time, shows the cytotoxic effect of the vapor phase of the volatile oil from L. dentata on lung tumor cell line Calu-3. The IC50 calculated for liquid phase of volatile oil was 388.84 μg.mL-1.The fraction of oil vapor in a concentration of 750 μg.mL-1 reduced the cell viability of Calu-3 significantly, based on three different techniques, the MTT reduction assays, SRB and DNA-PI. The results of morphological evaluations by Rosenfeld dyes, acridine orange/ethidium bromide and Hoescht, as well as the lactate dehydrogenase release assay gave an indication that the oil is cytotoxic to induce the cells death by necrosis, which was confirmed by the fact that the oil does not generate phosphatidylserine externalization. However, due to reports in literature, the expression of P-glycoprotein, which confers drug resistance in tumors and block apoptosis, in Calu-3 cells can not confirm the absence of this mechanism as well. The oil vapor promoted cell cycle arrest in G0/G1 when the DNA content was evaluated by flow cytometry. Considering these results, additional studies may be recommended, in order to use the volatile oil L. dentata as material for the pharmaceutical industry, with particular emphasis on the use of oil vapor in the lung cancer treatment therapy via inhalation.