Estudo in vitro da eficácia de antissépticos contra clones hospitalares de Acinetobacter baumannii

Detalhes bibliográficos
Ano de defesa: 2017
Autor(a) principal: Botelho, Erlen Cristina
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Estadual de Maringá
Brasil
Programa de Pós-Graduação em Ciências da Saúde
UEM
Maringá, PR
Centro de Ciências da Saúde
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Link de acesso: http://repositorio.uem.br:8080/jspui/handle/1/2048
Resumo: The resistance of hospital isolates of Acinetobacter baumannii to antibiotics has been frequently described in literature; however, few studies have investigated the resistance of this microorganism to antiseptics. To evaluate the efficacy of the main antiseptics used for hand hygiene against multidrug-resistant hospital isolates of Acinetobacter baumannii. The possible association between antibiotic and antiseptic resistance was also assessed. The quantitative suspension test performed in the presence of interfering substances as described in the 13727 European Standard method (EN 13727) was used to investigate the bactericidal activity of 2% chlorhexidine, 1% povidone-iodine, 70% w/w ethyl alcohol and 70% v/v ethyl alcohol gel against different multidrug-resistant hospital clones of Acinetobacter baumannii (N = 33). As a minimum requirement for the bactericidal activity the tested product shall demonstrate at least a 5 log10 reduction (for alcoholic preparations) or at least a 3 log10 reduction (for chlorhexidine and povidone-iodo) in the suspension test. All isolates of Acinetobacter baumannii were sensitive to the antiseptics tested under clean conditions (bovine albumin solution 1.5%) or dirty (15% bovine albumin solution plus 15% sheep erythrocytes). The mean±sd of bactericidal action of the tested products, expressed by the logarithmic reduction factor, in the clean and dirty conditions, were, respectively, 7.40±0.20 and 7.36±0.53 (chlorhexidine); 7.36±0.46 and 7.37 ±0.41 (povidone-iodine); 7.46±0.20 and 7.42±0.32 (ethyl alcohol); 7.40±0.32 and 7.48±0.12 (alcohol gel); demonstrating that each product tested fulfilled EN 13727. We conclude that all hospital isolates of Acinetobacter baumannii were killed by commonly used antiseptics and hand hygiene should be effective in limiting their spread in hospitals. Association between resistance to antibiotics and a decreased susceptibility to antiseptics was not found.