Avaliação da administração crônica de vitamina E sobre neurônios produtores de óxido nítrico no sistema nervoso central de ratos diabéticos

• Ano de defesa: 2008
• Autor(a) principal: Comin, Débora
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual de Maringá; Brasil; Programa de Pós-Graduação em Ciências Farmacêuticas; UEM; Maringá, PR; Departamento de Farmácia e Farmacologia
• Palavras-chave em Português: Farmacologia; Diabetes; Sistema nervoso central; Óxido nítrico; Labirinto em cruz elevado; Vitamina E; Brasil.; Streptozotocin; Elevated plus maze trial 1/2; NADPHdiaphorase; Vitamin E; Brazil.; Ciências da Saúde; Farmácia
• Link de acesso: http://repositorio.uem.br:8080/jspui/handle/1/1956

In the present study, we investigated the effect of chronic vitamin E administration on nitric oxide (NO)-producing neurons in the brain of streptozotocin (STZ)-induced diabetic rats. We further evaluated the effect of diabetes and vitamin E treatment on experimentally anxiety and memory processes using the elevated plus maze (EPM) Trial 1/2 protocol. Fifty adult Wistar rats were divided into four groups: normoglycemics (N), normoglycemics treated with vitamin E (NVE), diabetics (D) and diabetics treated with vitamin E (DVE). Diabetes mellitus was induced by a single intravenous injection of STZ (35 mg/kg). VE (100 mg/Kg) or vehicle, were administered by oral route (gavage, 1 ml/Kg) everyday during 7 weeks. After behavioral testing, the brains of the animals were removed and the striatum, paraventricular nucleus of hypothalamus (PVN), supraoptic nucleus (SON) and dorsolateral periaqueductal grey matter (DLPAG) were analysed for NADPHdiaphorase histochemistry. STZ-diabetic rats presented a decreased locomotor activity and cognitive impairments when compared to its normoglycemic controls in the EPM Trial 1/2 test. A significant increase in the soma area of NADPH-d positive neurons was detected in the striatum, PVN, SON and DLPAG while the number of NADPH-d positive neurons was increased only in the striatum and PAG of STZdiabetic rats. Vitamin E chronic treatment decreased the soma area of NADPH-d neurons in the PVN and SON. It also improved the cognitive deficits observed in STZ-diabetic rats.