Inclusão de bactérias probióticas em ração para gatos

• Ano de defesa: 2018
• Autor(a) principal: Rodrigues, Bruna Moura
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual de Maringá; Brasil; Programa de Pós-Graduação em Zootecnia; Maringá, PR; Centro de Ciências Agrárias
• Palavras-chave em Português: Felinos; Microbiota fecal; Microencapsulação; Shelf life - Vida de prateleira; Ciências Agrárias; Zootecnia
• Link de acesso: http://repositorio.uem.br:8080/jspui/handle/1/4712

The objective of this study was to evaluate the resistance of a commercial probiotic composed of Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus lactis, Bifidobacterium bifidum, Enterococcus faecium and Sacharomyces cerevisiae, to the microencapsulation process by the lyophilization and spray dryer technique, the modulation of the fecal microbiota of cats, as well as the microbial viability when added to commercial feed for a period of 120 days. For the lyophilization process, gum acacia, fructooligosaccharide and an association of the two were used as encapsulating agents, for spray dryer technique it was only the fructooligosaccharide. The microcapsules and the commercial product were also tested for in vitro passage through the gastrointestinal tract, being subjected to acidic conditions followed by alkaline conditions. Microbiological analyzes on MRS agar for lactic acid bacteria, M17 agar for Enterococcus faecium and YEPD agar for Sacharomyces cerevisiae were performed to determine their viability after processing. For the determination of the fecal microbiota modulation, three experimental treatments were used: T1 (control) commercial ration, T2 commercial ration with addition of probiotic and T3 commercial ration with addition of probiotic + fructooligosaccharide. The rations were given to 18 animals being, six experimental units per treatment, for a period of 21 days. The cats were housed in digestibility cages for feces collection and received during the experimental period feeding twice a day. The feces collected were analyzed for fecal score, pH, lactic acid bacteria (on MRS agar) and enterobacteria (on McConkey agar). For microbial viability to the shelf-life of the ration, feed samples were stored at room temperature for microbiological analyzes. A significant difference (P <0.05) was observed between the viability of the commercial product before and after simulation of the TGI, with counts of lactic acid bacteria, enterococci and yeasts, in log10/g of 8.25; 8.27; 8.25 and 7.28; 7.22; 7.18 respectively. When compared to the microencapsulation processes, lyophilization was more efficient, with the greater viability observed in the fructooligosaccharide-coated microcapsules,with 8.74 log10/g for lactic acid bacteria and 8.75 log10/g for enterococci, after in vitro digestibility there was a marked reduction in the count of lyophilized microorganisms (3.67 and 4.65 log10/g, respectively). For the Spray dryer technique there were not observed microorganisms due to injuries caused by the drying temperature. When added to the feed, it was possible to observe a significant reduction (P <0.05) in the viability of the microorganisms throughout the shelf-life of the food, but even with this reduction, there was a significant difference (P <0.05) in the modulation of fecal microbiota, since there was an increase in Lactobacillus count in treatments with addition of probiotic and probiotic plus fructooligosaccharide (5.07, 4.87, respectively) when compared to control (3.65 log10/g). It was verified that the inclusion of probiotic additive in the diet of cats was able to modulate the intestinal microbiota in a positive way, but due to the reduction in the microbial viability throughout shelf-life, it is necessary to use techniques such as microencapsulation, as well as the microbial resistance to the processing must be observed