Detalhes bibliográficos
| Ano de defesa: |
2003 |
| Autor(a) principal: |
Lopes, Cláudio Afonso Pinho |
| Orientador(a): |
Não Informado pela instituição |
| Banca de defesa: |
Não Informado pela instituição |
| Tipo de documento: |
Dissertação
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Estadual do Ceará
|
| Programa de Pós-Graduação: |
Não Informado pela instituição
|
| Departamento: |
Não Informado pela instituição
|
| País: |
Não Informado pela instituição
|
| Palavras-chave em Português: |
|
| Link de acesso: |
https://siduece.uece.br/siduece/trabalhoAcademicoPublico.jsf?id=27397
|
Resumo: |
Este trabalho teve como objetivo avaliar o potencial da imunização com extrato total de complexos <em>cumulus</em>-oócito suínos (EpCCO) para a esterilização de fêmeas de camundongos e de cadelas. Folículos pré-ovulatórios de ovários suínos foram puncionados para obtenção dos complexos <em>cumulus</em>-oócitos, que foram homogeneizados para produção do EpCCO. Camundongas foram imunizadas com doses de 100, 500, 1.000 ou 1.500 <span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:SimSun;">µ</span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:SimSun;"></span>g de EpCCO, enquanto que cadelas foram imunizadas com 10 mg de EpCCO. A produção de anticorpos anti-EpCCO foi avaliada por ELISA para as camundongas e por IDGA para as cadelas. A resposta imuine celular foi avaliada nas camundongas através da reação de hipersensibilidade tardia (RHT) após desafio com EpCCO. Ovários de metade das camundongas foram obtidos para exame histopatológico. A outra metade foi mantida com camundongos para avaliação do desempenho reprodutivo. A resposta imune ao EpCCO foi significativamente maior nos animais imunizados com 100 <span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:SimSun;">µ</span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:SimSun;"></span>g, reduzindo-se com o aumento da dose de imunização. Os escores de ELISA e a RHT foram significativamente maiores no grupo de animais imunizados com 100 <span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:SimSun;">µ</span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:SimSun;"></span>g (D100) e mais baixos no grupo imunizados com 1500 <span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:SimSun;">µ</span><span style="mso-spacerun:'yes';font-family:Calibri;mso-fareast-font-family:SimSun;"></span>g (D1500). Observou-se uma diminuição siginificativa dos foliculos antrais nos ovários dos animais de D100, enquanto em D1500 não houve alteração ovariana. Houve uma redução significativa do tamanho da ninhada no D100, enquanto em D1500 este parâmetro não se alterou. Houve produção de anticorpos anti-EpCCO nas cadelas imunizadas. 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Priority="99" SemiHidden="false" Name="Light Shading Accent 2" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Light List Accent 2" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Light Grid Accent 2" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium Shading 1 Accent 2" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium Shading 2 Accent 2" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium List 1 Accent 2" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium List 2 Accent 2" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium Grid 1 Accent 2" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium Grid 2 Accent 2" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium Grid 3 Accent 2" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Dark List Accent 2" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Colorful Shading Accent 2" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Colorful List Accent 2" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Colorful Grid Accent 2" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Light Shading Accent 3" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Light List Accent 3" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Light Grid Accent 3" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium Shading 1 Accent 3" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium Shading 2 Accent 3" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium List 1 Accent 3" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium List 2 Accent 3" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium Grid 1 Accent 3" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium Grid 2 Accent 3" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium Grid 3 Accent 3" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Dark List Accent 3" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Colorful Shading Accent 3" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Colorful List Accent 3" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Colorful Grid Accent 3" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Light Shading Accent 4" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Light List Accent 4" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Light Grid Accent 4" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium Shading 1 Accent 4" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium Shading 2 Accent 4" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium List 1 Accent 4" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium List 2 Accent 4" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium Grid 1 Accent 4" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium Grid 2 Accent 4" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium Grid 3 Accent 4" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Dark List Accent 4" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Colorful Shading Accent 4" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Colorful List Accent 4" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Colorful Grid Accent 4" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Light Shading Accent 5" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Light List Accent 5" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Light Grid Accent 5" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium Shading 1 Accent 5" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium Shading 2 Accent 5" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium List 1 Accent 5" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium List 2 Accent 5" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium Grid 1 Accent 5" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium Grid 2 Accent 5" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium Grid 3 Accent 5" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Dark List Accent 5" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Colorful Shading Accent 5" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Colorful List Accent 5" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Colorful Grid Accent 5" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Light Shading Accent 6" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Light List Accent 6" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Light Grid Accent 6" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium Shading 1 Accent 6" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium Shading 2 Accent 6" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium List 1 Accent 6" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium List 2 Accent 6" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium Grid 1 Accent 6" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium Grid 2 Accent 6" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Medium Grid 3 Accent 6" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Dark List Accent 6" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Colorful Shading Accent 6" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Colorful List Accent 6" ></w:LsdException> <w:LsdException Locked="false" Priority="99" SemiHidden="false" Name="Colorful Grid Accent 6" ></w:LsdException> </w:LatentStyles></xml><![endif]--> |
