Estudo químico e avaliação das atividades biológicas dos galhos de Piranhea trifoliata BAILL (PICRODENDRACEAE)

• Ano de defesa: 2014
• Autor(a) principal: Pedroza, Laila da Silva
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade do Estado do Amazonas; Brasil; UEA; Programa de Pós-Graduação em Biotecnologia e Recursos Naturais
• Palavras-chave em Português: Picrodendraceae; Piranhea trifoliata; Fitoquímica; Biotecnologia
• Link de acesso: https://ri.uea.edu.br/handle/riuea/2051

The aim of this work was to realize the chemical study of branches of Piranhea trifoliata (Picrodendraceae) and evaluate the biological activity as antimalarial, antioxidant, antibacterial activities and toxicityabout Artemia salina. The plant material was collected three times, two in Para State and one in Amazon State. The material was dried and extracted using dichromethane and methanol as solvents. There were realized TLC analyzes of these two extracts (CH2Cl2 and MeOH) and were observed characteristics of terpenes after derivatization with anisadehyde and Ce(SO4)2. The phytochemical study of CH2Cl2 extract from the second collection yielded 3-oxo-friedelin. The antimalarial activity of the extracts and fases from MeOH extract were tested against Plasmodium falciparum. The MeOH extracts from first (IC50 = 13.66 µg/mL) and second collection (IC50 = 5.79 µg/mL) and CH2Cl2 fase (IC50 = 7.05 µg/mL) were active, this fase was evaluated for its cytotoxicity on the cell line HepG2 A16 (derived from a primary human hepatoblastoma) and concentration reduction was greater than 1000 mg/mL and after calculated the selectivity index, was not considered toxic and so it was possible to infer that this fase has a directed only to the parasite and not effect that this action is due to its toxicity for all types of cells. To antioxidant activity of the MeOH extracts of third collection has equivalence with ascorbic acid at 1-2. To antimicrobial activity by agar diffusion method, the MeOH extract of the 2nd collection has activity against the bacteria Salmonella enteritidis e Pseudomonas fluorescens, with inhibition halos of 1.5 cm and 1.3 cm. This is the first report of 3-oxo-friedelin in Picrodendraceae Keywords: Picrodendraceae, Piranhea trifoliata, phytochemical