Modelos Estruturais in Silico e Express??o de Quimeras da Gonadotrofina Cori??nica Equina

• Ano de defesa: 2014
• Autor(a) principal: Amaral, F??bio de Barros
• Orientador(a): Barbosa, Jo??o Alexandre Ribeiro Gon??alves , Parachin, N??dia Skorupa
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Cat??lica de Bras??lia
• Programa de Pós-Graduação: Programa Strictu Sensu em Ci??ncias Gen??micas e Biotecnologia
• Departamento: Escola de Sa??de e Medicina
• País: Brasil
• Palavras-chave em Português: Gen??tica; Genoma; Gonadotrofina Cori??nica Equina (eCG); Horm??nio; Modelagem.; Horm??nio Fol??culo Estimulante (FSH); Horm??nio Luteinizante (LH)
• Área do conhecimento CNPq: CIENCIAS BIOLOGICAS::GENETICA
• Resumo em Inglês: The equine chorionic gonadotropin (eCG) has been used as an important resource in the processes of reproduction in animals of economic interest, especially horses and cattle. The eCG molecule consists of a glycoprotein composed of two subunits called alpha and beta which together perform the function of both LH (luteinizing hormone) and FSH (follicle stimulating hormone) and due to this characteristic has great potential to induce ovulation in household species. Based on the aforementioned characteristics, the present work was was aimed at the study and characterization of the structure of eCG using molecular modeling techniques as well as testing its production in cells of the microorganism Pichia pastoris. Furthermore, chimeric models have been proposed linking the eCG and immunoglobulin G (IgG) molecule based on studies that reveal the great potential of it in raising the half-life of other molecules circulating in the organism. Four chimeric models were prepared so that the eCG alpha and beta chains are connected by a hinge, short and flexible regions, while the terminal region of the alpha chain was connected to the N-terminal portion of the CH2 domain of the IgG by another hinge region, thus resulting in a model in which two different molecules are linked.
• Link de acesso: https://bdtd.ucb.br:8443/jspui/handle/tede/1999

The equine chorionic gonadotropin (eCG) has been used as an important resource in the processes of reproduction in animals of economic interest, especially horses and cattle. The eCG molecule consists of a glycoprotein composed of two subunits called alpha and beta which together perform the function of both LH (luteinizing hormone) and FSH (follicle stimulating hormone) and due to this characteristic has great potential to induce ovulation in household species. Based on the aforementioned characteristics, the present work was was aimed at the study and characterization of the structure of eCG using molecular modeling techniques as well as testing its production in cells of the microorganism Pichia pastoris. Furthermore, chimeric models have been proposed linking the eCG and immunoglobulin G (IgG) molecule based on studies that reveal the great potential of it in raising the half-life of other molecules circulating in the organism. Four chimeric models were prepared so that the eCG alpha and beta chains are connected by a hinge, short and flexible regions, while the terminal region of the alpha chain was connected to the N-terminal portion of the CH2 domain of the IgG by another hinge region, thus resulting in a model in which two different molecules are linked.