Detalhes bibliográficos
| Ano de defesa: |
2017 |
| Autor(a) principal: |
Modesto, Karina Ribeiro
 |
| Orientador(a): |
Pereira, Rinaldo Wellerson
|
| Banca de defesa: |
Não Informado pela instituição |
| Tipo de documento: |
Dissertação
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Cat??lica de Bras??lia
|
| Programa de Pós-Graduação: |
Programa Strictu Sensu em Ci??ncias Gen??micas e Biotecnologia
|
| Departamento: |
Escola de Sa??de e Medicina
|
| País: |
Brasil
|
| Palavras-chave em Português: |
|
| Área do conhecimento CNPq: |
|
| Resumo em Inglês: |
Recent discoveries points towards seminal plasma playing a major role in directing processes on mammalian spermatozoa as well as influencing offspring development prior to and after birth. Amongst the candidates for such role, recent data has brought the extracellular vesicles (EVs) into the field. Aiming to characterize EVs present on the intraluminal fluid of different segments of the epididymis, such as cauda and caput in male Wistar rats, this research assessed the influence of sexual developmental stages on the concentration and size distribution of epididymal EVs. The individuals were also grouped in terms of sexual developmental stages, clustered as ???prior to puberty, ???puberty???, and ???post-puberty???. TRPS based analysis showed a significant shifts in concentration and diameter of such EVs between clustering groups as well as epididymal segment groups. Caput epididymal EVs were found presenting reduced concentration and reduced diameter when compared to cauda epididymis EVs. Regarding the more sexually developed individuals, those showed higher EVs counts than ???prior to puberty??? ones. Additional findings suggest that post-puberty epididymal EVs are enlarged in comparison to the two other groups. Such data might serve a purpose of better comprehending the processes regarding spermatic maturation and capacitation (fertilization), offering findings that bring up clinically important issues regarding male epigenesis and father-to-offspring effects. |
| Link de acesso: |
https://bdtd.ucb.br:8443/jspui/handle/tede/2270
|
Resumo: |
Recent discoveries points towards seminal plasma playing a major role in directing processes on mammalian spermatozoa as well as influencing offspring development prior to and after birth. Amongst the candidates for such role, recent data has brought the extracellular vesicles (EVs) into the field. Aiming to characterize EVs present on the intraluminal fluid of different segments of the epididymis, such as cauda and caput in male Wistar rats, this research assessed the influence of sexual developmental stages on the concentration and size distribution of epididymal EVs. The individuals were also grouped in terms of sexual developmental stages, clustered as ???prior to puberty, ???puberty???, and ???post-puberty???. TRPS based analysis showed a significant shifts in concentration and diameter of such EVs between clustering groups as well as epididymal segment groups. Caput epididymal EVs were found presenting reduced concentration and reduced diameter when compared to cauda epididymis EVs. Regarding the more sexually developed individuals, those showed higher EVs counts than ???prior to puberty??? ones. Additional findings suggest that post-puberty epididymal EVs are enlarged in comparison to the two other groups. Such data might serve a purpose of better comprehending the processes regarding spermatic maturation and capacitation (fertilization), offering findings that bring up clinically important issues regarding male epigenesis and father-to-offspring effects. |
