Análise comparativa in vitro da citotoxicidade dos ácidos hialurônicos de alto e baixo peso molecular em enxertia óssea

Detalhes bibliográficos
Ano de defesa: 2014
Autor(a) principal: Bordin, Thaisa Barizan lattes
Orientador(a): Teixeira, Eduardo Rolim lattes
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Pontifícia Universidade Católica do Rio Grande do Sul
Programa de Pós-Graduação: Programa de Pós-Graduação em Odontologia
Departamento: Faculdade de Odontologia
País: BR
Palavras-chave em Português:
Área do conhecimento CNPq:
Link de acesso: http://tede2.pucrs.br/tede2/handle/tede/1237
Resumo: In recent decades, tissue engineering techniques have been reported in the literature as an alternative to conventional autogenous grafts for bone defects and subsequent installation of dental implants, combining a material with the same biological properties of autogenous bone without its limiting factors. This technology requires a carrier for the cells, where hyaluronic acid, a natural glycosaminoglycan, appears to have suitable characteristics to carry out this role. It exerts various biological functions depending on its molecular weight, which can be classified as high (> 1000 kDa), low (from 50 to 1000 kDa) or very low (<50 kDa). Furthermore, there is the hyaluronidase, an enzyme that degrades hyaluronic acid into smaller fragments, influencing the cellular response. Thus, this study aimed to evaluate in vitro the influence of the enzyme hyaluronidase (HYAL) on the high hyaluronic acid (HA-HMW) and low molecular weight (HA-LMW) and their potential cytotoxicity on NIH - 3T3 cells. The MTT assay was performed analyzing cell viability of the following groups: (G1) Cells + HA-HMW; (G2) Cells + HA- LMW; (G3) Cells + HA-HMW + HYAL; (G4) Cells + HA-LMW + HYAL; (G5) Cells + HA-HMW + hydroxyapatite; (G6) Cells + HA-LMW + hydroxyapatite; (G7) Cells + HA-HMW + HYAL + hydroxyapatite; (G8) Cells + HA-LMW + HYAL + hydroxyapatite; (G9) Cells (positive control); (G10) Cells + hypochlorite (negative control). The cell viability for each group were as follows: (G1) 76,0%, (G2) 63,3%, (G3) 67,4%, (G4) 76,5%, (G5) 57,6%, (G6) 60,8%, (G7) 64,4%, (G8) 74,3%, (G9) 100% e (G10) 12,4%. It was found that all groups had significantly higher cell viability compared to the negative control. And although there was no significant difference between experimental groups, only the G1, G4 and G8 groups did not differ significantly from the positive control. Therefore, HA-HMW had better viability in the absence of enzyme hyaluronidase, although HA-LMW provided greater cell viability in the presence of the enzyme. Results indicated that the low-weight hyaluronic acid might be applicable for tissue engineering grafting techniques.