Análise de biomarcadores circulantes modulados pela terapia de fotobiomodulação em indivíduos com trombose venosa profunda

Detalhes bibliográficos
Ano de defesa: 2020
Autor(a) principal: Brigidio, Eduardo Alves lattes
Orientador(a): Silva Junior, Jose Antonio lattes
Banca de defesa: Silva Junior, Jose Antonio lattes, Zamuner, Stella Regina lattes, Chavantes, Maria Cristina Chavantes lattes, Baltatu, Ovidiu Constantin lattes
Tipo de documento: Tese
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Nove de Julho
Programa de Pós-Graduação: Programa de Mestrado em Medicina
Departamento: Saúde
País: Brasil
Palavras-chave em Português:
Palavras-chave em Inglês:
Área do conhecimento CNPq:
Link de acesso: http://bibliotecatede.uninove.br/handle/tede/2754
Resumo: Venous thromboembolism (VTE) is a disease with high morbidity and mortality that includes pulmonary embolism (PE) and deep vein thrombosis (DVT). Deep vein thrombosis is a common disease and has little specific characteristics, which often makes its diagnosis difficult. Photobiomodulation therapy (PBM) has important anti-inflammatory effects such as reduction of chemical mediators such as cytokines, increased generation of nitric oxide (NO) and decreased migration of inflammatory cells. Changes in microRNA expression have recently been reported after PBM therapy. The aim of the project was to analyze the expression transcriptional elements such as messenger RNA (mRNA) and circulating microRNAs in subjects with DVT after irradiation with FBM therapy. Twenty subjects with subacute thrombosis in femoropopliteal territory and 10 healthy subjects as control were studied. DVT subjects were randomly separated into 2 groups of 10 individuals. Of these, only one group received PBM therapy. PBM therapy was applied at wavelength 660 nm. The peripheral blood of all subjects was collected before and after ultrasound and irradiation. The biomarker analyzes were performed with Real-Time PCR, in addition to Western blot analysis and measurement of the total NO concentration. We observed that individuals with DVT have lower expression of eNOS mRNA, higher expression of IL-6 and reduced concentration of NO compared to healthy individuals. However, after PBM therapy, subjects with DVT increased eNOS expression, decreased IL-6 expression, increased total NO concentration and modulated expression of circulating biomarkers such as miR-195 and mir-338. This is the first time that the effect of PBM therapy has been observed in subjects with subacute deep venous thrombosis, modulating transcriptional elements that can contribute to the management of the disease.