Avaliação da atividade de diterpenos frente a bactérias multirresistentes

• Ano de defesa: 2011
• Autor(a) principal: Silva, José Rodolfo Macedo Souza da
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade de Franca; Brasil; Pós-Graduação; Programa de Mestrado em Ciências; UNIFRAN
• Palavras-chave em Português: Química - Produtos naturais (Viguiera arenaria); Copaifera langsdorffii - Mikania glomerata, diterpenos; Ácido ent-pimara-8(14), 15-dien-19-óico; Ent-8(14),15-pimaradien-3ß-ol; Ácido ent-caur-16(17)-en-19-óico; ácido ent-8(17)-13e-labdadieno-15-óico; Atividade biológica; bactéria multirresistentes; CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA
• Link de acesso: https://repositorio.cruzeirodosul.edu.br/handle/123456789/516

In the last years, microbial resistance to the multiple drugs, as well as the appearance of new types of infections is increasing rapidly and becoming a work-wide public health problem (Gaspar-Marques et al. 2006; Spellberg et al., 2004) because they are infectious diseases a major cause of morbidity and mortality in developed and developing countries (Ahmad & Beg, 2001). Before the appearance of infections with microorganisms resistant strains to conventional chemotherapy, the scientific community has been over the years focusing to find a new sources of substances to be use against multiresistant bacteria. One of the main sources of these substances are natural products, by isolation of essential metabolites for its maintenance, which are commonly applied in several studies of biological activity. Among these metabolites used, the diterpenes has shown promising results, individually or as enhancers of other substances present on the market. Natural products biosynthesized from mevalonic acid through the pyrophosphate 2E, 6E, 10E-geranilgeranila (GGPP) (Dewick, 2002). According to the number of rings and the cyclization pattern of their chemical structures, diterpenes are divided into acyclic, bicyclic, tricyclic, tetracyclic, and mixed macrocyclic (Garcia et al, 2007; Hanson, 2004). The dissertation in question sought to isolate through Viguiera arenaria strata, Copaifera langsdorffii and Mikania glomerata diterpenes ent-pimara-8 (14) ,15-dien-19-oic acid, ent8 (14)-3β ,15-pimaradien -ol, ent-cowrie-16 (17)-en-19-oic acid and ent-8 (17)-13Elabdadieno-15-oic acid, which after the isolation were subjected to analysis of 13C NMR (Nuclear Magnetic Resonance Nuclear Carbon 13) and 1H NMR (Nuclear Magnetic Resonance of Hydrogen) to determinate their chemical structure. After elucidated the diterpenes chemical structures mentioned above, its potential has been tested front of bacterias Staphylococcus aureus - IC WB81 USA 400, Staphylococcus aureus - 180 HCRP, Staphylococcus aureus - ATCC 29213, Staphylococcus aureus - IC W7749 USA 200, Staphylococcus capitis - ATCC 27840 , Staphylococcus capitis - 207 HCRP, Staphylococcus epidermis - ATCC 14990, Staphylococcus epidermis - 177 HCRP, Staphylococcus haemolyticus - 213 HCRP, Staphylococcus haemolyticus ATCC 29970, Streptococcus pneumoniae – 176 HCRP, Enterococcus faecalis - 179 HCRP, Enterococcus faecalis - NCTC 775, Enterococcus faecium - NCTC 717 in the Laboratory Research in Applied Microbiology (LAPEMA - Unifran), under the supervision of Professor. Dr. Carlos Henrique Gomes Martins. During the determination of minimum inhibitory concentration, we found promising results against a panel of multiresistant bacteria of relevant clinical importance based on the values proposed by Ríos & Recio.