Estudo da influência do L-PRP no reparo ósseo e desenvolvimento medular por análise da imunoexpressão de proteínas e marcadores moleculares

• Ano de defesa: 2019
• Autor(a) principal: Göhringer, Isabella
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Positivo; Brasil; Pós-Graduação; Programa de Pós-Graduação em Odontologia Clínica; UP
• Palavras-chave em Português: Odontologia; Ossos - regeneração; CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
• Link de acesso: https://repositorio.cruzeirodosul.edu.br/handle/123456789/2171

Aim: The aim of this study was to evaluate the role of L-PRP during bone repair and medullary differentiation in artificial defect in mice calvary treated and not treated with L-PRP through the analysis of the immunohistochemical expression of TGF-β, osteoprotegerin, osteocalcin, sclerotin, CD34, IGF-1, JAK2, STAT5, IP3R and SMA. Methods: A bone defect was created on the calvaria of 48 wisteria mice. They were divided into four groups (L-PRP + particulate bone autograft and only particulate bone autograft; 15 and 40 days euthaniasia) and treated according to their group graft. The animals were euthanized 15 and 40 days postoperatively. The analysis of the results was performed through immunohistochemistry interpretations. Results: The results of this research revealed a decrease in bone matrix formation, and a change in medullary differentiation in L-PRP treated groups. Conclusion: The presence of L-PRP impaired bone repair and produced changes in medullary differentiation, demonstrating conditions similar to pathological fibrotic processes.