Avaliação da imunoexpressão da osteoprotegerina, RANKL e IP3R no reparo ósseo craniofacial tratados com autoenxerto na presença de alendronato em ratas uterosalpingo-ovariectomizadas

• Ano de defesa: 2019
• Autor(a) principal: Sant’Ana, Renan Dainez de
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Positivo; Brasil; Pós-Graduação; Programa de Pós-Graduação em Odontologia Clínica; UP
• Palavras-chave em Português: Odontologia; Transplante autólogo; Osteogênese; Regeneração óssea; Anormalidades craniofaciais; CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
• Link de acesso: https://repositorio.cruzeirodosul.edu.br/handle/123456789/2092

Bisphosphonates are drugs known to be modulators of bone remodeling, with alendronate as its most widely used agent. Its mode of action is the modulation of the mevalonate-cholesterol pathway, being the inhibitor of osteoclastogenesis and favorable to osteogenesis. The objective of this study was to evaluate the immunoexpresion of the IP3R protein, as well as the analysis between OPG / RANKL in critical defects filled with particulate autogenous bone, besides the quantification of the bone matrix present in the defects in 60 ovariectomized utero-salpingo adult rats in the presence or absence of administration of 1mg / kg of sodium alendronate. After 15 and 60 days, euthanasia and histological, histomorphometric and immunohistochemical results were performed. The results obtained demonstrated a greater bone quantification of the Alendronate group in 60 days. The Rank-L showed greater expressiveness to non-control group, especially in 15 days, with the value increasing based on bone matrix deposition. The OPG AND IP3R, unlike Rank-L, presented themselves noticeably higher in the specimens treated with Alendronate, more specifically in the peripheral areas of the pattern and areas surrounding the autograft. The results coincided with increased bone deposition in specimens receiving alendronate. Within the limitations of this study, it was concluded that the use of Alendronate increases osteoconductivity in craniofacial defects, in addition to increasing the reference level between OPG / RANKL, besides the greater expression of IP3R, an important regulator of OPG.