Estudo fitoquímico de Styrax camporum Pohl (Styracaceae) e avaliação das atividades esquistossomicida, inibitória da enzima ciclooxigenase e sequestradora de radicais livres

• Ano de defesa: 2012
• Autor(a) principal: Braguine, Caio Guedes
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade de Franca; Brasil; Pós-Graduação; Programa de Mestrado em Ciências; UNIFRAN
• Palavras-chave em Português: Styrax camporum; Styracaceae; Atividade sequestradora de radicais livres; DPPH; Inibição da ciclooxigenase; Atividade esquistossomicida; Flavonóides; CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA
• Link de acesso: https://repositorio.cruzeirodosul.edu.br/handle/123456789/513

The species Styrax camporum Pohl, popularly known as "estoraque do campo", "cuia de brejo" and "benjoeiro'', is used in folk medicine to treat gastrointestinal diseases. This study aimed to identify natural compounds from the extract of S. camporum and evaluateschistosomicidal, COX inhibition, and radical scavenging activities. The phytochemical study of the bioactive EtOAc fraction, obtained from the crude extract liquid-liquid partition, by High Performance Liquid Chromatography, resulted in the isolation of two flavonoids, quercetin (1) and kaempferol (2), which were described for the first time in S. camporum. The partial fractions obtained in the liquid-liquid partition inhibit COX-1 and 2 at a concentration of 10 µg/ml and showed moderate antioxidant activity. The pairs of adult worms incubated with the fractions AcOEt, n-BuOH and n-hexane at a concentration of 100 µg/mL, caused a decrease in motor activity and separation of couples. Only the n-hexane fraction caused death of the parasites (25 %) and extensive changes in the adult worms tegument. Considering the antischistosomal activity of flavonoids isolated in this work, worms incubated with 1 (100 µM) had a moderate decreasein motor activity, without changes in the tegument. Flavonoid2 showed IC50 values of S. mansonicouple separation of 25.0 µM at 120 h. However, only the flavonoid 2 was capable of causing death of 25% of the worms at 100 µM at 120 h.