Avaliação dos efeitos do extrato bruto de levedura Sporobolomyces ruberrimus em ratos

• Ano de defesa: 2014
• Autor(a) principal: Zoz, Liliana Isabel Chitolina
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Positivo; Brasil; Pós-Graduação; Programa de Pós-Graduação em Biotecnologia Industrial; UP
• Palavras-chave em Português: Torularodina; Carotenoides; Sporobolomyces ruberrimus; Toxicidade; Antioxidante; CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
• Link de acesso: https://repositorio.cruzeirodosul.edu.br/handle/123456789/2579

The carotenoids pigments are molecules of high commercial value because of its biological properties and its application on the most different branch of industry, like food coloring, pharmaceutical supplements and cosmetics. The base of its production has been biotechnological, aiming the achievement of a natural product, different from others synthetics that don’t add nutritional value to the product in question. Torularhodin is a carotenoid pigment that shows, in vitro, potential antioxidant activity higher than the most known of them, β−caroteno, being synthesized by red yeasts, but meanly by Sporobolomyces ruberrimus. There is no study found, until now, that verify the tissues effects of these pigments, in vivo, in the form of brute extract, that was the aim of this study. The utilization of fresh biomass of Sporobolomyces ruberrimus could minimize the extraction’s costs and purification of pigments produced by this yeast, as compared with the carotenoids produced nowadays. For this study was utilized 50 animals females, and 50 animals males, that were separated on groups and that received five different dosages (0 mg, 0,5 mg, 1,0 mg, 1,5 mg, 2,0 mg of pigments/ Kg of weight of animal/ per day during 60 days). The animals were euthanized for analysis of tissues as: liver, kidneys, esophagi, stomach and intestine. It was observed alteration on the tissues, especially in intestinal tissue, occurring an increase of Peyer’s patch, when compared to control group. In stomach tissue was observed ulcerations. It’s evidenced by this study that the use of biomass of Sporobolomyces ruberrimus in the form of crude extract is not suitable for consumption, suggesting that the extraction and purification of carotenoid pigments produced by it should be made.