Efeitos do 7,12- dimetilbenzetraceno como fator carcinogênico em ratas da linhagem Wistar

• Ano de defesa: 2014
• Autor(a) principal: Dossena, Grazziele Matte
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Positivo; Brasil; Pós-Graduação; Programa de Pós-Graduação em Biotecnologia Industrial; UP
• Palavras-chave em Português: Mamas - Tumores; Pulmões - Tumores; Carcinogênese; Imunohistoquímica; CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
• Link de acesso: https://repositorio.cruzeirodosul.edu.br/handle/123456789/2582

Mortality by cancer is the second most common cause in the worldwide. The more necessity of knowledge about epidemiological data and better understanding of tumor biology drive researchers to study cancer trying to better perceive the evolution of that disease. In the early stages of the studies about carcinogesis, rodents may be required as preclinical models. The models have been made out by inoculation of tumor cells or by administration of carcinogenic chemical agents for inducing tumors. The carcinogenic chemicals are usually carcinogens belonging to the family of polycyclic aromatic hydrocarbons, highlighting the 7,12 – dimetilbenzetraceno (DMBA). Therefore, the aim of this study had been develop pattern models of carcinogenesis in female Wistar rats, induced by DMBA carcinogenic chemical agent. Moreover, the goal had been investigate which DMBA dosing can be used to create the pattern model and evaluate cell proliferation, apoptosis and hormonal. To that purpose, 55 rats were used to create the pattern model. The rats were divided into four groups according to the dose of the inducing agent applied. To tumor induction were applied DMBA in 45 rats and the dosages were 3; 6 and 9 mg/kg. The control group were consisted by ten animals and they have received just corn oil. All the tissues of interest were collected and were evaluted by histopathological and immunohistochemistry with Ki-67, p53, C-erb-B2, estrogen receptor and progesterone receptor exams. In all groups evaluated only the groups that have received 6 and 9 mg/kg of DMBA have developed tumor in breast, lung and trachea and the group that have received 9 mg/kg showed more injuries due to toxicity in the liver and kidneys. Patterns using C-erb-B2, p53, estrogen receptor, progesterone receptor and Ki-67 were considered negative for most of the tissues evaluated and some positive immunostaining for C-erb-B2, p53 and estrogen receptor were detected in groups that received 3 and 6 mg/kg of DMBA. It can be concluded that the dosage of 6 mg/kg of DMBA have demonstrated a innovative appearance and incidence of lung tumors in Wistar rats.