Avaliação histológica e imuno-histoquímica da expressão de Wnt3a e vimentina na aplicação ectópica em ovelhas de matriz óssea bovina inorgânica associada a extrato experiemental de nácar

• Ano de defesa: 2017
• Autor(a) principal: Spada, Gilvan Robson
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Positivo; Brasil; Programa de Pós-Graduação; Programa de Pós-Graduação em Odontologia Clínica; UP
• Palavras-chave em Português: Odontologia; Matriz óssea; Biomaterial; Nácar; Wnt3a; CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
• Link de acesso: https://repositorio.cruzeirodosul.edu.br/handle/123456789/2055

The search for biomaterials, or their association, capable of promoting osteoconduction and osteoinduction, without causing an unwanted inflammatory reaction and being biocompatible, remains a challenging and current theme. The present study had the objective of evaluating the tissue response inducing the ectopic graft of the inorganic bovine bone matrix (IBM) associated with a fluid extract of marine nacre (ME) in dorsal muscular region of sheep, by histological and immunohistochemical analysis. Eight sheep, monitored for 3 (n = 4) and 6 months (n = 4), received three polyethylene tubes with the following characteristics: empty tube (Sham group); tube with pure IBM; and tube with IBM associated with ME. After the times mentioned, and collection of muscle samples with grafts, histological analysis revealed the presence of typical bone connective tissue around the particles of IBM, and the presence of a mature bone tissue was more evident where the particles were associated with ME. Regarding immunohistochemistry, Vimentin, marker of fibroblastic activity, did not present differences between the tubes tested. However, regarding Wnt3a, a marker of osteoblastic differentiation, there was a decrease in its activity in the presence of IBM in 6 months, especially when associated with ME (p <0.05). It can be concluded that the association IBM + ME presented a greater potential of bone induction, being that this cellular stimulus responsible for the differentiation in osteoblasts was different from the local connective tissue response by fibroblastos.