Produção de celulose bacteriana por scoby de kombucha em comparação à bactéria Gluconacetobacter xylinus

• Ano de defesa: 2022
• Autor(a) principal: Rigoni, Carolina
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Positivo; Brasil; Pós-Graduação; Programa de Pós-Graduação em Biotecnologia Industrial; UP
• Palavras-chave em Português: Curativo; Hidrogel; Bactéria acética; CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
• Link de acesso: https://repositorio.cruzeirodosul.edu.br/handle/123456789/3801

Bacterial cellulose has compared to plant cellulose a high degree of purity, mechanical resistance, crystallinity, biocompatibility and high-water absorption, presenting potential application in several areas. It can be produced by several microbial sources, such as the Gluconacetobacter xylinus bacteria, with a high level consists of a microbial community made up of yeasts and bacteria. However, thehigh cost of producing bacterial cellulose compromises its production on an industrial scale. Culture media that use alternative carbon sources have potential for production, reducing the cost of the final product. The objective of the present work was to evaluate the production of bacterial cellulose in static culture using SCOBY from kombucha and G. xylinus in a medium containing glycerol as a carbon source. BC production using SCOBY and the isolated microorganism G. xylinus was performed in 400 mL of HSG medium at 26 °C for 7 days, for 5 consecutive weeks. After production, the membranes were purified by treatment with NaOH and acetic acid and subsequently dried for 24 hours at 45 °C, to determine the dry mass. Then, the methods commonly used for characterization of bacterial cellulose were carried out: yield, microbiological control through the sterility test, tensile strength, water absorption and resistance to basic and acidic solutions. The production using the microorganism G. xylinus obtained a yield like that obtained using SCOBY in the fifth week of cultivation, of 1,64 g/L and 1,48 g/L, respectively. The sterility test showed that the purification step was able to remove viable cells, as well as the autoclaving process, since no medium turbidity was observed in any of the samples. Among the parameters evaluated, there was no statistical difference, considering a 95% confidence interval, in the membranes produced by G. xylinus and SCOBY for the mechanical resistance and acid resistance tests. However, for the resistance test to basic solutions and for the water absorption test, it was verified that the membrane from G. xylinus was able to absorb more water and there was a greater loss of mass in the basic solution test. The results of this work demonstrate that SCOBY from kombucha presents itself as a potential source of bacterial cellulose both in terms of production yield and characteristics of the membrane obtained. New studies should be carried out seeking to investigate the use of alternative carbon sources for its production.