Avaliação de polimorfismos genéticos associados ao bruxismo

• Ano de defesa: 2019
• Autor(a) principal: Savaris, Leonardo Brunet
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Positivo; Brasil; Pós-Graduação; Programa de Pós-Graduação em Odontologia Clínica; UP
• Palavras-chave em Português: Odontologia; Bruxismo; Polimorfismo (Genética); CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
• Link de acesso: https://repositorio.cruzeirodosul.edu.br/handle/123456789/2100

Introduction: Bruxism is the habit of tooth grinding or clenching while awake and sleeping with at least one sign or symptom. Although bruxism impacts quality of life and is highly prevalent in children and adolescents, its intrinsic etiologic factors (i.e genetic factors) have not been extensively studied in this population yet. Objective: This study was to determine the association of bruxism and circadian manifestations with specific genes that can be helpful to understand the etiology of this disorder in children and adolescents. Methods: One hundred and fifty subjects (7 – 12 years old) were included in this case-control study. DNA was collected from buccal epithelial cells to determine the association between bruxism and FKBP5 (rs1360780 and rs3800373), DRD2 (rs6275 and rs6276), ANKK1 (rs1800497) and COMT (rs6269). Genes polymorphism were investigated through RT-PCR with the TaqMan™ assay in a StepOnePlus System. Logistic univariate regression was used to associate genotypes under additive and dominant models between cases and controls, with significance level of 0.05. Results: In the diagnosis of bruxism, the DRD2 (rs6276) is associated with a higher prevalence in GG homozygous individuals when compared to AA or GA subjects [p = 0.02 / 2.47 (1.13 - 5.36)]. In relation to circadian manifestations of bruxism, the polymorphism ANKK1 is associated with awake teeth grinding (p < 0.001). The DRD2 (rs6276) associated with grinding teeth during sleep in the additive genotypes (p = 0.03) and dominant (p = 0.008). Concerning awake tooth clenching , it had a prevalence in the ANKK1 gene of the TT genotype in both additive (p = 0.05) and dominant (p = 0.08) modes. In sleep tooth clenching , a prevalence of COMT genes in the GG model was found for both additive (p = 0.003) and dominant (p = 0.001). Concerning the aweke tooth clenching, there was association with TT individuals for the ANKK1 gene in the additive model (p = 0.05) and association with the ANKK1 gene (p = 0.08) in the dominant model with the TT gene (p = 0.08). In sleep tooth clenching, was found in the COMT genes in the GG model for both additive (p = 0.003) and dominant (p = 0.001).Conclusion: The DRD2 gene polymorphism is related to bruxism; and ANKK1 and COMT are associated with circadian manifestation of bruxism