Isolamento, obtenção por semi-síntese e avaliação da atividade antimicrobiana de diterpenos de Mikania glomerata e Copaifera langsdorffii frente a bactérias cariogênicas

• Ano de defesa: 2011
• Autor(a) principal: Rocha, Erilda Marques Pereira da
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade de Franca; Brasil; Pós-Graduação; Programa de Doutorado em Ciências; UNIFRAN
• Palavras-chave em Português: Diterpenos; Mikania glomerata; Copaifera langsdorffii; Atividade antimicrobiana; Bactérias cariogênicas; CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA
• Link de acesso: https://repositorio.cruzeirodosul.edu.br/handle/123456789/785

Caries is the main pathology affecting the oral cavity. This bacterial disease results in dissolution of tooth enamel and, if not stopped, it can lead to demineralization of dental tissues and consequently to tooth loss. This study proposes to obtain and evaluate the antimicrobial activity of diterpenes isolated from Mikania glomerata and Copaifera langsdorffii and synthetic analogues against cariogenic bacteria. The antimicrobial activity was performed using the broth microdilution method for determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) and through the Time kill curve of the most active diterpenes against the main cariogenic microorganism (Streptococcus mutans). The metabolites with higher antimicrobial activity were kaurenoic acid, its respective salt and copalic acid, showing significant activity (MIC <10µg/mL). Considering the values of MBC and the similarity in the Time kill curve profile, copalic acid showed the best results against the main cariogenic bacteria.