Efeito imunomodulatório da suplementação preventiva do ácido graxo ômega-3 em um modelo animal da Síndrome da Dor Regional Complexa do tipo I

• Ano de defesa: 2020
• Autor(a) principal: Fernandes, Paula Franson
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Palavras-chave em Português: Ômega-3; PUFAs; Síndrome da Dor Regional Complexa; Edema; Citocinas
• Link de acesso: https://repositorio.animaeducacao.com.br/handle/ANIMA/2996

Introduction: Complex Regional Pain Syndrome (CRPS) is known to present autonomic, sensory, motor and trophic clinical manifestations, triggered by a trauma or surgical process, and is established after an inflammatory process followed by the injury. It presents an increase of pro-inflammatory cytokines, and a decrease of anti-inflammatory and pro-resolutive cytokines. Omega-3, a fish oil rich in eicosapentaenoic acid (EPA) and docosaexaenoic acid (DHA), is known for its anti-inflammatory and pro-resolutive properties. Therefore, the objective of this study was to verify the effect of the preventive supplementation with omega-3 on the paw edema, in the concentrations of interleukin (IL)-4, IL-10, transforming growth factor β-1 (TGF-β1), tumor necrosis factor (TNF) and monocyte chemoattractant protein-1 (MCP-1) in the skin and paw muscle and finally in the immunocontent of NOS 2 and CD86 (macrophages M1) and Arginase-1 (macrophages M2) in the paw muscle of mice submitted to the model of Chronic PostIschemia Pain (CPIP). Methodology: Swiss female mice were divided into 4 groups: (1) Saline/Sham; (2) Saline/CPIP; (3) Corn oil/CPIP and (4) Omega-3/CPIP. Saline (0.9%), corn oil (1.500 mg/Kg) or omega-3 (1.500 mg/Kg) were added for 30 days preventively and then, according to the two experimental protocols, until the 2nd or 15th day after CPIP. The CPIP model was performed by applying a tourniquet on the posterior right paw of the mice, inducing a process of ischemia and reperfusion. The evaluation of the leg thickness was used to follow the edema up to 96 hours after the injury. ELISA was used to measure pro- and anti-inflammatory cytokines and the Western Blotting assay for NOS 2 and CD86 and Arginase-1 immunomarkers. Results: Omega-3 decreased paw edema in injured animals, increased IL-4 concentrations in muscle after 48 hours, and increased IL-10 concentrations 15 days after IDP. The omega-3 did not change the concentrations of MCP-1, TNF and TGF-β1.48 hours after CPIP, as well as it did not change the levels of NOS 2, CD86 and Arginase-1. Conclusion: The results demonstrate a consistent immunomodulatory effect of preventive omega-3 supplementation in an animal model of CRPS-I.