Avaliação da citotoxicidade de solução de ácido hipocloroso obtida a partir de dispositivo eletrolítico – estudo in vitro
محفوظ في:
| المؤلف الرئيسي: | |
|---|---|
| تاريخ النشر: | 2023 |
| التنسيق: | Bachelor thesis |
| اللغة: | por |
| المصدر: | Repositório Institucional da UPF |
| Download full: | https://repositorio.upf.br/handle/123456789/7408 |
الملخص: | The objective of the present study was to evaluate, in vitro, the cytotoxicity of a hypochlorous acid solution obtained from an electrolytic device, at a concentration of 250 ppm, in comparison with sodium hypochlorite at a concentration of 2.5%. The cytotoxicity test was performed using the MTT assay, which corresponds to a colorimetric test used to assess cell viability. The groups used to carry out this study were: G1 – distilled water (control); G2 – 2.5% sodium hypochlorite (2.5% NaOCl) and G3 – 250ppm hypochlorous acid (250 ppm HClO). After preparing the cell culture medium, 100 uL of the same treatment groups already mentioned were added individually to the wells containing the medium, being incubated for a period of 3 minutes. All experiments were performed in triplicate and cell viability percentages were calculated relative to cell controls. Cytotoxicity was analyzed by one-way ANOVA followed by Tukey's post-hoc test (α=0.05). The results of the cytotoxicity test revealed that all chemical substances tested were statistically different when compared to the control group. However, the G3 group was less cytotoxic than the G2 group. It can be concluded that hypochlorous acid was less cytotoxic when compared to sodium hypochlorite, in addition to being as effective as bacterial reduction. |
مواد مشابهة: Avaliação da citotoxicidade de solução de ácido hipocloroso obtida a partir de dispositivo eletrolítico – estudo in vitro
- Influência do ácido hipocloroso obtido a partir de um dispositivo eletrolítico na resistência flexural da dentina radicular – estudo in vitro
- Avaliação da ação antimicrobiana do ácido hipocloroso obtido a partir de dispositivo eletrolítico em canais radiculares infectados com enterococcus faecalis
- Influência do hipoclorito de cálcio na resistência de união de materiais restauradores à dentina coronária
- Influência do ácido hipocloroso obtido a partir de um dispositivo eletrolítico nas propriedades da dentina radicular: estudo in vitro
- Avaliação da efetividade antimicrobiana de diferentes agentes químicos na eliminação de Enterococcus faecalis e Candida albicans
- Potencial antimicrobiano da associação de óleos essenciais Tea Tree (Melaleuca alternifolia) e Petitgrain (citrus aurantium) contra microrganismos presentes no sistema de canais radiculares : Estudo in vitro