Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae)
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| Hlavní autor: | |
|---|---|
| Datum vydání: | 2000 |
| Další autoři: | , , , , |
| Médium: | Article |
| Jazyk: | por |
| Zdroj: | Repositório Institucional da UnB |
| Download full: | http://repositorio.unb.br/handle/10482/25609 https://dx.doi.org/10.1590/S0100-879X2000000800005 |
Shrnutí: | We describe here the isolation and characterization of a major albumin from the seeds of Cereus jamacaru (Cactaceae), to which we gave the trivial name of cactin. This protein has a molecular mass of 11.3 kDa and is formed by a light chain (3.67 kDa) and a heavy chain (7.63 kDa). This protein was isolated using a combination of gel filtration chromatography and reverse-phase HPLC. The amino acid composition of cactin was determined and found to resemble that of the 2S seed reserve protein from the Brazil nut, a protein remarkable for its high methionine content. The usefulness of cactin as a molecular marker in the taxonomy of the Cactaceae is discussed. |
| _version_ | 1871442617141559296 |
|---|---|
| author | Aragão, Theresa Christine Filgueiras Russo |
| author2 | Souza, Petronio Augusto Simão de Uchôa, Adriana Ferreira Costa, Itayguara Ribeiro da Bloch Júnior, Carlos Campos, Francisco de Assis de Paiva |
| author2_role | author author author author author |
| author_browse | Aragão, Theresa Christine Filgueiras Russo Bloch Júnior, Carlos Campos, Francisco de Assis de Paiva Costa, Itayguara Ribeiro da Souza, Petronio Augusto Simão de Uchôa, Adriana Ferreira |
| author_facet | Aragão, Theresa Christine Filgueiras Russo Souza, Petronio Augusto Simão de Uchôa, Adriana Ferreira Costa, Itayguara Ribeiro da Bloch Júnior, Carlos Campos, Francisco de Assis de Paiva |
| author_role | author |
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| bitstream.checksumAlgorithm.fl_str_mv | MD5 |
| bitstream.url.fl_str_mv | http://repositorio.unb.br/bitstream/10482/25609/1/ARTIGO_CharacterizationMethionineRich.pdf |
| collection | Repositório Institucional da UnB |
| dc.contributor.author.fl_str_mv | Aragão, Theresa Christine Filgueiras Russo Souza, Petronio Augusto Simão de Uchôa, Adriana Ferreira Costa, Itayguara Ribeiro da Bloch Júnior, Carlos Campos, Francisco de Assis de Paiva |
| dc.contributor.email.pt_BR.fl_str_mv | mailto:bioplant@ufc.br |
| dc.date.accessioned.fl_str_mv | 2017-12-07T04:32:53Z |
| dc.date.available.fl_str_mv | 2017-12-07T04:32:53Z |
| dc.date.issued.fl_str_mv | 2000 |
| dc.identifier.citation.fl_str_mv | ARAGÃO, T. C. F. R. et. al. Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae). Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 33, n. 8, p. 897-903, ago. 2000. DOI 10.1590/S0100-879X2000000800005. Disponível em: https://www.scielo.br/j/bjmbr/a/bDbfzsyZW6fsPvhXMNdbTtt/?lang=en. Acesso em: 17 fev. 2025. |
| dc.identifier.doi.pt_BR.fl_str_mv | https://dx.doi.org/10.1590/S0100-879X2000000800005 |
| dc.identifier.uri.fl_str_mv | http://repositorio.unb.br/handle/10482/25609 |
| dc.language.iso.fl_str_mv | por |
| dc.publisher.none.fl_str_mv | Associação Brasileira de Divulgação Científica |
| dc.rights.driver.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositório Institucional da UnB instname:Universidade de Brasília (UnB) instacron:UNB |
| dc.subject.keyword.pt_BR.fl_str_mv | Cacto Mandacarú Cereus jamacaru Metionina |
| dc.title.pt_BR.fl_str_mv | Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae) |
| dc.type.driver.fl_str_mv | info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | We describe here the isolation and characterization of a major albumin from the seeds of Cereus jamacaru (Cactaceae), to which we gave the trivial name of cactin. This protein has a molecular mass of 11.3 kDa and is formed by a light chain (3.67 kDa) and a heavy chain (7.63 kDa). This protein was isolated using a combination of gel filtration chromatography and reverse-phase HPLC. The amino acid composition of cactin was determined and found to resemble that of the 2S seed reserve protein from the Brazil nut, a protein remarkable for its high methionine content. The usefulness of cactin as a molecular marker in the taxonomy of the Cactaceae is discussed. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | UNB_f2e8e4a59c2e76becc1490debb309993 |
| identifier_str_mv | ARAGÃO, T. C. F. R. et. al. Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae). Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 33, n. 8, p. 897-903, ago. 2000. DOI 10.1590/S0100-879X2000000800005. Disponível em: https://www.scielo.br/j/bjmbr/a/bDbfzsyZW6fsPvhXMNdbTtt/?lang=en. Acesso em: 17 fev. 2025. |
| instacron_str | UNB |
| institution | UNB |
| instname_str | Universidade de Brasília (UnB) |
| language | por |
| network_acronym_str | UNB |
| network_name_str | Repositório Institucional da UnB |
| oai_identifier_str | oai:repositorio.unb.br:10482/25609 |
| publishDate | 2000 |
| publishDateSort | 2000 |
| publisher.none.fl_str_mv | Associação Brasileira de Divulgação Científica |
| reponame_str | Repositório Institucional da UnB |
| repository.mail.fl_str_mv | repositorio@unb.br |
| repository.name.fl_str_mv | Repositório Institucional da UnB - Universidade de Brasília (UnB) |
| repository_id_str | |
| spelling | Aragão, Theresa Christine Filgueiras RussoSouza, Petronio Augusto Simão deUchôa, Adriana FerreiraCosta, Itayguara Ribeiro daBloch Júnior, CarlosCampos, Francisco de Assis de Paivamailto:bioplant@ufc.br2017-12-07T04:32:53Z2017-12-07T04:32:53Z2000ARAGÃO, T. C. F. R. et. al. Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae). Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 33, n. 8, p. 897-903, ago. 2000. DOI 10.1590/S0100-879X2000000800005. Disponível em: https://www.scielo.br/j/bjmbr/a/bDbfzsyZW6fsPvhXMNdbTtt/?lang=en. Acesso em: 17 fev. 2025.http://repositorio.unb.br/handle/10482/25609https://dx.doi.org/10.1590/S0100-879X2000000800005Associação Brasileira de Divulgação CientíficaCharacterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCactoMandacarúCereus jamacaruMetioninaWe describe here the isolation and characterization of a major albumin from the seeds of Cereus jamacaru (Cactaceae), to which we gave the trivial name of cactin. This protein has a molecular mass of 11.3 kDa and is formed by a light chain (3.67 kDa) and a heavy chain (7.63 kDa). This protein was isolated using a combination of gel filtration chromatography and reverse-phase HPLC. The amino acid composition of cactin was determined and found to resemble that of the 2S seed reserve protein from the Brazil nut, a protein remarkable for its high methionine content. The usefulness of cactin as a molecular marker in the taxonomy of the Cactaceae is discussed.Instituto de Química (IQ)info:eu-repo/semantics/openAccessporreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNBORIGINALARTIGO_CharacterizationMethionineRich.pdfARTIGO_CharacterizationMethionineRich.pdfapplication/pdf210881http://repositorio.unb.br/bitstream/10482/25609/1/ARTIGO_CharacterizationMethionineRich.pdfe655fbce56af73385c9510343e5d39fbMD51open access10482/256092025-02-17 17:27:27.428open accessoai:repositorio.unb.br:10482/25609Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2025-02-17T20:27:27Repositório Institucional da UnB - Universidade de Brasília (UnB) |
| spellingShingle | Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae) Aragão, Theresa Christine Filgueiras Russo Cacto Mandacarú Cereus jamacaru Metionina |
| status_str | publishedVersion |
| title | Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae) |
| title_full | Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae) |
| title_fullStr | Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae) |
| title_full_unstemmed | Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae) |
| title_short | Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae) |
| title_sort | Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae) |
| topic | Cacto Mandacarú Cereus jamacaru Metionina |
| url | http://repositorio.unb.br/handle/10482/25609 https://dx.doi.org/10.1590/S0100-879X2000000800005 |