Characterization of a methionine-rich protein from the seeds of Cereus jamacaru Mill. (Cactaceae)
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| Main Author: | |
|---|---|
| Publication Date: | 2000 |
| Other Authors: | , , , , |
| Format: | Article |
| Language: | por |
| Source: | Repositório Institucional da UnB |
| Download full: | http://repositorio.unb.br/handle/10482/25609 https://dx.doi.org/10.1590/S0100-879X2000000800005 |
Summary: | We describe here the isolation and characterization of a major albumin from the seeds of Cereus jamacaru (Cactaceae), to which we gave the trivial name of cactin. This protein has a molecular mass of 11.3 kDa and is formed by a light chain (3.67 kDa) and a heavy chain (7.63 kDa). This protein was isolated using a combination of gel filtration chromatography and reverse-phase HPLC. The amino acid composition of cactin was determined and found to resemble that of the 2S seed reserve protein from the Brazil nut, a protein remarkable for its high methionine content. The usefulness of cactin as a molecular marker in the taxonomy of the Cactaceae is discussed. |
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