Melatonin prevents age-related hearing loss in the murin experimental model
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| Main Author: | |
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| Publication Date: | 2022 |
| Other Authors: | , , , , , |
| Format: | Article |
| Language: | eng |
| Source: | Repositório Institucional da UnB |
| Download full: | https://repositorio.unb.br/handle/10482/45209 https://doi.org/10.1016/j.bjorl.2022.06.002 https://orcid.org/0000-0002-8113-7222 https://orcid.org/0000-0001-7853-3016 https://orcid.org/0000-0003-4494-3353 https://orcid.org/0000-0002-2777-9494 https://orcid.org/0000-0001-5132-8370 https://orcid.org/0000-0001-7677-772X https://orcid.org/0000-0001-7611-1303 |
Summary: | Objective: The present study aimed to perform a morphological and morphometric analysis of cochlear structures of C57BL/6J mice receiving oral melatonin for a 12-month period. Methods: 32 male C57BL/6J were divided into control and melatonin groups. Control received saline and ethanol solution and melatonin group, 50 L of 10 mg of melatonin/kg/day orally for a 12-month period. After de experiment the animals were sacrificed into a 40% concentration of CO2 chamber, and the blades were morphological and morphometrically analyzed. Results: The melatonin group revealed a higher median density of viable cells (45 ± 10.28 cells/100 m2, 31---73, vs. 32 ± 7.47 cells/100 m2, 25---48). The median area of stria vascularis was 55.0 ± 12.27 cells/100 m2 (38---80) in the control, and 59.0 ± 16.13 cells/100 m2 (40---134) in the melatonin group. The morphometric analysis of the spiral ligament reveals a higher median of total viable neurons in the melatonin (41 ± 7.47 cells/100 m2, 27---60) than in the control group (31 ± 5.68 cells/100 m2, 21---44). Conclusion: Although melatonin is a potent antioxidant, it does not completely neutralize the occurrence of presbycusis; however, it may delay the onset of this condition. |
| Summary: | Objective: The present study aimed to perform a morphological and morphometric analysis of cochlear structures of C57BL/6J mice receiving oral melatonin for a 12-month period. Methods: 32 male C57BL/6J were divided into control and melatonin groups. Control received saline and ethanol solution and melatonin group, 50 L of 10 mg of melatonin/kg/day orally for a 12-month period. After de experiment the animals were sacrificed into a 40% concentration of CO2 chamber, and the blades were morphological and morphometrically analyzed. Results: The melatonin group revealed a higher median density of viable cells (45 ± 10.28 cells/100 m2, 31---73, vs. 32 ± 7.47 cells/100 m2, 25---48). The median area of stria vascularis was 55.0 ± 12.27 cells/100 m2 (38---80) in the control, and 59.0 ± 16.13 cells/100 m2 (40---134) in the melatonin group. The morphometric analysis of the spiral ligament reveals a higher median of total viable neurons in the melatonin (41 ± 7.47 cells/100 m2, 27---60) than in the control group (31 ± 5.68 cells/100 m2, 21---44). Conclusion: Although melatonin is a potent antioxidant, it does not completely neutralize the occurrence of presbycusis; however, it may delay the onset of this condition. |
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