Implementação de um sistema de eletroforese capilar com detecção de fluorescência induzida por laser
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| 主要作者: | |
|---|---|
| Publication Date: | 2000 |
| 其他作者: | , |
| 格式: | Article |
| 语言: | por |
| Source: | Repositório Institucional da UnB |
| Download full: | http://repositorio.unb.br/handle/10482/25599 https://dx.doi.org/10.1590/S0100-40422000000500003 |
总结: | A capillary electrophoresis system using laser induced fluorescence detection is described. A Raman system equipped with a microscope has been used to focus the laser beam on the capillary giving a lateral resolution of 1.5 mm. The fluorescence signal of the analyte (ZnPcTS - tetrasulfonated zinc-phthalocyanine) was collected by the microscope objectives and analysed by a monochromator with confocal characteristics equipped with a CCD detector. Electropherograms obtained with this system were compared to those obtained on a commercial instrument, showing that the described system presents a lower detection limit and better resolution. |