Implementação de um sistema de eletroforese capilar com detecção de fluorescência induzida por laser
Պահպանված է:
| Հիմնական հեղինակ: | |
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| Հրապարակման ամսաթիվ: | 2000 |
| Այլ հեղինակներ: | , |
| Ձևաչափ: | Article |
| Լեզու: | por |
| Աղբյուր: | Repositório Institucional da UnB |
| Download full: | http://repositorio.unb.br/handle/10482/25599 https://dx.doi.org/10.1590/S0100-40422000000500003 |
Ամփոփում: | A capillary electrophoresis system using laser induced fluorescence detection is described. A Raman system equipped with a microscope has been used to focus the laser beam on the capillary giving a lateral resolution of 1.5 mm. The fluorescence signal of the analyte (ZnPcTS - tetrasulfonated zinc-phthalocyanine) was collected by the microscope objectives and analysed by a monochromator with confocal characteristics equipped with a CCD detector. Electropherograms obtained with this system were compared to those obtained on a commercial instrument, showing that the described system presents a lower detection limit and better resolution. |
| Ամփոփում: | A capillary electrophoresis system using laser induced fluorescence detection is described. A Raman system equipped with a microscope has been used to focus the laser beam on the capillary giving a lateral resolution of 1.5 mm. The fluorescence signal of the analyte (ZnPcTS - tetrasulfonated zinc-phthalocyanine) was collected by the microscope objectives and analysed by a monochromator with confocal characteristics equipped with a CCD detector. Electropherograms obtained with this system were compared to those obtained on a commercial instrument, showing that the described system presents a lower detection limit and better resolution. |
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