Molecular characterization of Hovenia dulcis-associated virus 1 (HDaV1) and 2 (HDaV2) : new tentative species within the order picornavirales
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| Autore principale: | |
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| Data di pubblicazione: | 2020 |
| Altri autori: | , , , , , , , |
| Natura: | Article |
| Lingua: | eng |
| Fonte: | Repositório Institucional da UnB |
| Download full: | https://repositorio.unb.br/handle/10482/39576 https://doi.org/10.3390/v12090950 https://orcid.org/0000-0002-5791-8002 https://orcid.org/0000-0002-4965-1424 |
Riassunto: | In a systematic field survey for plant-infecting viruses, leaf tissues were collected from trees showing virus-like symptoms in Brazil. After viral enrichment, total RNA was extracted and sequenced using the MiSeq platform (Illumina). Two nearly full-length picorna-like genomes of 9534 and 8158 nucleotides were found associated with Hovenia dulcis (Rhamnaceae family). Based upon their genomic information, specific primers were synthetized and used in RT-PCR assays to identify plants hosting the viral sequences. The larger contig was tentatively named as Hovenia dulcis-associated virus 1 (HDaV1), and it exhibited low nucleotide and amino acid identities with Picornavirales species. The smaller contig was related to insect-associated members of the Dicistroviridae family but exhibited a distinct genome organization with three non-overlapping open reading frames (ORFs), and it was tentatively named as Hovenia dulcis-associated virus 2 (HDaV2). Phylogenetic analysis using the amino acid sequence of RNA-dependent RNA polymerase (RdRp) revealed that HDaV1 and HDaV2 clustered in distinct groups, and both viruses were tentatively assigned as new members of the order Picornavirales. HDaV2 was assigned as a novel species in the Dicistroviridae family. The 50 ends of both viruses are incomplete. In addition, a nucleotide composition analysis (NCA) revealed that HDaV1 and HDaV2 have similarities with invertebrate-infecting viruses, suggesting that the primary host(s) of these novel virus species remains to be discovered. |