Molecular characterization of Hovenia dulcis-associated virus 1 (HDaV1) and 2 (HDaV2) : new tentative species within the order picornavirales
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| Autor principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | eng |
| Fonte: | Repositório Institucional da UnB |
| Texto Completo: | https://repositorio.unb.br/handle/10482/39576 https://doi.org/10.3390/v12090950 https://orcid.org/0000-0002-5791-8002 https://orcid.org/0000-0002-4965-1424 |
Resumo: | In a systematic field survey for plant-infecting viruses, leaf tissues were collected from trees showing virus-like symptoms in Brazil. After viral enrichment, total RNA was extracted and sequenced using the MiSeq platform (Illumina). Two nearly full-length picorna-like genomes of 9534 and 8158 nucleotides were found associated with Hovenia dulcis (Rhamnaceae family). Based upon their genomic information, specific primers were synthetized and used in RT-PCR assays to identify plants hosting the viral sequences. The larger contig was tentatively named as Hovenia dulcis-associated virus 1 (HDaV1), and it exhibited low nucleotide and amino acid identities with Picornavirales species. The smaller contig was related to insect-associated members of the Dicistroviridae family but exhibited a distinct genome organization with three non-overlapping open reading frames (ORFs), and it was tentatively named as Hovenia dulcis-associated virus 2 (HDaV2). Phylogenetic analysis using the amino acid sequence of RNA-dependent RNA polymerase (RdRp) revealed that HDaV1 and HDaV2 clustered in distinct groups, and both viruses were tentatively assigned as new members of the order Picornavirales. HDaV2 was assigned as a novel species in the Dicistroviridae family. The 50 ends of both viruses are incomplete. In addition, a nucleotide composition analysis (NCA) revealed that HDaV1 and HDaV2 have similarities with invertebrate-infecting viruses, suggesting that the primary host(s) of these novel virus species remains to be discovered. |
| _version_ | 1871442305036058624 |
|---|---|
| author | Nery, Flávia M. B. |
| author2 | Melo, Fernando Lucas Boiteux, Leonardo Silva Ribeiro, Simone G. Resende, Renato de Oliveira Orílio, Anelise Franco Batista, Josiane Goulart Lima, Mirtes Freitas Carvalho, Rita de Cássia Pereira |
| author2_role | author author author author author author author author |
| author_browse | Batista, Josiane Goulart Boiteux, Leonardo Silva Carvalho, Rita de Cássia Pereira Lima, Mirtes Freitas Melo, Fernando Lucas Nery, Flávia M. B. Orílio, Anelise Franco Resende, Renato de Oliveira Ribeiro, Simone G. |
| author_facet | Nery, Flávia M. B. Melo, Fernando Lucas Boiteux, Leonardo Silva Ribeiro, Simone G. Resende, Renato de Oliveira Orílio, Anelise Franco Batista, Josiane Goulart Lima, Mirtes Freitas Carvalho, Rita de Cássia Pereira |
| author_role | author |
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| bitstream.url.fl_str_mv | http://repositorio2.unb.br/jspui/bitstream/10482/39576/2/license.txt http://repositorio2.unb.br/jspui/bitstream/10482/39576/1/ARTIGO_MolecularCharacterizationHoveniaDulcis.pdf |
| collection | Repositório Institucional da UnB |
| dc.contributor.author.fl_str_mv | Nery, Flávia M. B. Melo, Fernando Lucas Boiteux, Leonardo Silva Ribeiro, Simone G. Resende, Renato de Oliveira Orílio, Anelise Franco Batista, Josiane Goulart Lima, Mirtes Freitas Carvalho, Rita de Cássia Pereira |
| dc.date.accessioned.fl_str_mv | 2020-10-22T13:35:09Z |
| dc.date.available.fl_str_mv | 2020-10-22T13:35:09Z |
| dc.date.issued.fl_str_mv | 2020-08-27 |
| dc.identifier.citation.fl_str_mv | NERY, Flávia M. B. et al. Molecular characterization of Hovenia dulcis-associated virus 1 (HDaV1) and 2 (HDaV2): new tentative species within the order picornavirales. Viruses, v. 12, n. 9, 950, 2020. DOI: https://doi.org/10.3390/v12090950. Disponível em: https://www.mdpi.com/1999-4915/12/9/950. Acesso em: 22 out. 2020. |
| dc.identifier.doi.pt_BR.fl_str_mv | https://doi.org/10.3390/v12090950 |
| dc.identifier.orcid.pt_BR.fl_str_mv | https://orcid.org/0000-0002-5791-8002 https://orcid.org/0000-0002-4965-1424 |
| dc.identifier.uri.fl_str_mv | https://repositorio.unb.br/handle/10482/39576 |
| dc.language.iso.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | MDPI |
| dc.rights.driver.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositório Institucional da UnB instname:Universidade de Brasília (UnB) instacron:UNB |
| dc.subject.keyword.pt_BR.fl_str_mv | Vírus de plantas Metagenômica Picornavirales |
| dc.title.pt_BR.fl_str_mv | Molecular characterization of Hovenia dulcis-associated virus 1 (HDaV1) and 2 (HDaV2) : new tentative species within the order picornavirales |
| dc.type.driver.fl_str_mv | info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | In a systematic field survey for plant-infecting viruses, leaf tissues were collected from trees showing virus-like symptoms in Brazil. After viral enrichment, total RNA was extracted and sequenced using the MiSeq platform (Illumina). Two nearly full-length picorna-like genomes of 9534 and 8158 nucleotides were found associated with Hovenia dulcis (Rhamnaceae family). Based upon their genomic information, specific primers were synthetized and used in RT-PCR assays to identify plants hosting the viral sequences. The larger contig was tentatively named as Hovenia dulcis-associated virus 1 (HDaV1), and it exhibited low nucleotide and amino acid identities with Picornavirales species. The smaller contig was related to insect-associated members of the Dicistroviridae family but exhibited a distinct genome organization with three non-overlapping open reading frames (ORFs), and it was tentatively named as Hovenia dulcis-associated virus 2 (HDaV2). Phylogenetic analysis using the amino acid sequence of RNA-dependent RNA polymerase (RdRp) revealed that HDaV1 and HDaV2 clustered in distinct groups, and both viruses were tentatively assigned as new members of the order Picornavirales. HDaV2 was assigned as a novel species in the Dicistroviridae family. The 50 ends of both viruses are incomplete. In addition, a nucleotide composition analysis (NCA) revealed that HDaV1 and HDaV2 have similarities with invertebrate-infecting viruses, suggesting that the primary host(s) of these novel virus species remains to be discovered. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | UNB_4c8ede898baf5a2ee2bd0f9ae538e08f |
| identifier_str_mv | NERY, Flávia M. B. et al. Molecular characterization of Hovenia dulcis-associated virus 1 (HDaV1) and 2 (HDaV2): new tentative species within the order picornavirales. Viruses, v. 12, n. 9, 950, 2020. DOI: https://doi.org/10.3390/v12090950. Disponível em: https://www.mdpi.com/1999-4915/12/9/950. Acesso em: 22 out. 2020. |
| instacron_str | UNB |
| institution | UNB |
| instname_str | Universidade de Brasília (UnB) |
| language | eng |
| network_acronym_str | UNB |
| network_name_str | Repositório Institucional da UnB |
| oai_identifier_str | oai:repositorio.unb.br:10482/39576 |
| publishDate | 2020 |
| publishDateSort | 2020 |
| publisher.none.fl_str_mv | MDPI |
| reponame_str | Repositório Institucional da UnB |
| repository.mail.fl_str_mv | repositorio@unb.br |
| repository.name.fl_str_mv | Repositório Institucional da UnB - Universidade de Brasília (UnB) |
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| spelling | Nery, Flávia M. B.Melo, Fernando LucasBoiteux, Leonardo SilvaRibeiro, Simone G.Resende, Renato de OliveiraOrílio, Anelise FrancoBatista, Josiane GoulartLima, Mirtes FreitasCarvalho, Rita de Cássia Pereira2020-10-22T13:35:09Z2020-10-22T13:35:09Z2020-08-27NERY, Flávia M. B. et al. Molecular characterization of Hovenia dulcis-associated virus 1 (HDaV1) and 2 (HDaV2): new tentative species within the order picornavirales. Viruses, v. 12, n. 9, 950, 2020. DOI: https://doi.org/10.3390/v12090950. Disponível em: https://www.mdpi.com/1999-4915/12/9/950. Acesso em: 22 out. 2020.https://repositorio.unb.br/handle/10482/39576https://doi.org/10.3390/v12090950https://orcid.org/0000-0002-5791-8002https://orcid.org/0000-0002-4965-1424MDPI© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).info:eu-repo/semantics/openAccessMolecular characterization of Hovenia dulcis-associated virus 1 (HDaV1) and 2 (HDaV2) : new tentative species within the order picornaviralesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleVírus de plantasMetagenômicaPicornaviralesIn a systematic field survey for plant-infecting viruses, leaf tissues were collected from trees showing virus-like symptoms in Brazil. After viral enrichment, total RNA was extracted and sequenced using the MiSeq platform (Illumina). Two nearly full-length picorna-like genomes of 9534 and 8158 nucleotides were found associated with Hovenia dulcis (Rhamnaceae family). Based upon their genomic information, specific primers were synthetized and used in RT-PCR assays to identify plants hosting the viral sequences. The larger contig was tentatively named as Hovenia dulcis-associated virus 1 (HDaV1), and it exhibited low nucleotide and amino acid identities with Picornavirales species. The smaller contig was related to insect-associated members of the Dicistroviridae family but exhibited a distinct genome organization with three non-overlapping open reading frames (ORFs), and it was tentatively named as Hovenia dulcis-associated virus 2 (HDaV2). Phylogenetic analysis using the amino acid sequence of RNA-dependent RNA polymerase (RdRp) revealed that HDaV1 and HDaV2 clustered in distinct groups, and both viruses were tentatively assigned as new members of the order Picornavirales. HDaV2 was assigned as a novel species in the Dicistroviridae family. The 50 ends of both viruses are incomplete. In addition, a nucleotide composition analysis (NCA) revealed that HDaV1 and HDaV2 have similarities with invertebrate-infecting viruses, suggesting that the primary host(s) of these novel virus species remains to be discovered.engreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNBLICENSElicense.txtlicense.txttext/plain163http://repositorio2.unb.br/jspui/bitstream/10482/39576/2/license.txtba54f8d1c5f5ec8df897ad678916c701MD52open accessORIGINALARTIGO_MolecularCharacterizationHoveniaDulcis.pdfARTIGO_MolecularCharacterizationHoveniaDulcis.pdfapplication/pdf1887288http://repositorio2.unb.br/jspui/bitstream/10482/39576/1/ARTIGO_MolecularCharacterizationHoveniaDulcis.pdf8289b6b203b25c1396edd81d5caaaa92MD51open access10482/395762023-05-24 20:45:08.204open accessoai:repositorio.unb.br:10482/39576U3VibWlzc8OjbyBlZmV0aXZhZGEgcG9yIGludGVncmFudGUgZGEgZXF1aXBlIGRvIFJlcG9zaXTDs3JpbyBJbnN0aXR1Y2lvbmFsIGRhIFVuQiBkZSBhY29yZG8gY29tIGxpY2Vuw6dhIGNvbmNlZGlkYSBwZWxvIGF1dG9yIGUvb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLg==Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2023-05-24T23:45:08Repositório Institucional da UnB - Universidade de Brasília (UnB) |
| spellingShingle | Molecular characterization of Hovenia dulcis-associated virus 1 (HDaV1) and 2 (HDaV2) : new tentative species within the order picornavirales Nery, Flávia M. B. Vírus de plantas Metagenômica Picornavirales |
| status_str | publishedVersion |
| title | Molecular characterization of Hovenia dulcis-associated virus 1 (HDaV1) and 2 (HDaV2) : new tentative species within the order picornavirales |
| title_full | Molecular characterization of Hovenia dulcis-associated virus 1 (HDaV1) and 2 (HDaV2) : new tentative species within the order picornavirales |
| title_fullStr | Molecular characterization of Hovenia dulcis-associated virus 1 (HDaV1) and 2 (HDaV2) : new tentative species within the order picornavirales |
| title_full_unstemmed | Molecular characterization of Hovenia dulcis-associated virus 1 (HDaV1) and 2 (HDaV2) : new tentative species within the order picornavirales |
| title_short | Molecular characterization of Hovenia dulcis-associated virus 1 (HDaV1) and 2 (HDaV2) : new tentative species within the order picornavirales |
| title_sort | Molecular characterization of Hovenia dulcis-associated virus 1 (HDaV1) and 2 (HDaV2) : new tentative species within the order picornavirales |
| topic | Vírus de plantas Metagenômica Picornavirales |
| url | https://repositorio.unb.br/handle/10482/39576 https://doi.org/10.3390/v12090950 https://orcid.org/0000-0002-5791-8002 https://orcid.org/0000-0002-4965-1424 |