Genome-wide analysis of the transcriptional response to drought stress in root and leaf of common bean
Gardado en:
| Autor Principal: | |
|---|---|
| Data de Publicación: | 2019 |
| Outros autores: | , , , , , , , , , , |
| Formato: | Article |
| Idioma: | eng |
| Fonte: | Repositório Institucional da UnB |
| Download full: | https://repositorio.unb.br/handle/10482/36477 https://doi.org/10.1590/1678-4685-gmb-2018-0259 http://orcid.org/0000-0001-6954-6608 http://orcid.org/0000-0003-0644-5652 |
Summary: | Genes related to the response to drought stress in leaf and root tissue of drought-susceptible (DS) and tolerant (DT) genotypes were characterized by RNA-Seq. In total, 54,750 transcripts, representative of 28,590 genes, were identified; of these, 1,648 were of high-fidelity (merge of 12 libraries) and described for the first time in the Andean germplasm. From the 1,239 differentially expressed genes (DEGs), 458 were identified in DT, with a predominance of genes in categories of oxidative stress, response to stimulus and kinase activity. Most genes related to oxidation-reduction terms in roots were early triggered in DT (T75) compared to DS (T150) suggestive of a mechanism of tolerance by reducing the damage from ROS. Among the KEGG enriched by DEGs up-regulated in DT leaves, two related to the formation of Sulfur-containing compounds, which are known for their involvement in tolerance to abiotic stresses, were common to all treatments. Through qPCR, 88.64% of the DEGs were validated. A total of 151,283 variants were identified and functional effects estimated for 85,780. The raw data files were submitted to the NCBI database. A transcriptome map revealed new genes and isoforms under drought. These results supports a better understanding of the drought tolerance mechanisms in beans. |
| _version_ | 1871442294227337216 |
|---|---|
| author | Pereira, Wendell Jacinto |
| author2 | Melo, Arthur Tavares de Oliveira Coelho, Alexandre Siqueira Guedes Rodrigues, Fabiana Aparecida Caldart Mamidi, Sujan Alencar, Sérgio Amorim de Lanna, Anna Cristina Valdisser, Paula Arielle Mendes Ribeiro Brondani, Claudio Nascimento Júnior, Ivanildo Ramalho do Borba, Tereza Cristina de Oliveira Vianello, Rosana Pereira |
| author2_role | author author author author author author author author author author author |
| author_browse | Alencar, Sérgio Amorim de Borba, Tereza Cristina de Oliveira Brondani, Claudio Coelho, Alexandre Siqueira Guedes Lanna, Anna Cristina Mamidi, Sujan Melo, Arthur Tavares de Oliveira Nascimento Júnior, Ivanildo Ramalho do Pereira, Wendell Jacinto Rodrigues, Fabiana Aparecida Caldart Valdisser, Paula Arielle Mendes Ribeiro Vianello, Rosana Pereira |
| author_facet | Pereira, Wendell Jacinto Melo, Arthur Tavares de Oliveira Coelho, Alexandre Siqueira Guedes Rodrigues, Fabiana Aparecida Caldart Mamidi, Sujan Alencar, Sérgio Amorim de Lanna, Anna Cristina Valdisser, Paula Arielle Mendes Ribeiro Brondani, Claudio Nascimento Júnior, Ivanildo Ramalho do Borba, Tereza Cristina de Oliveira Vianello, Rosana Pereira |
| author_role | author |
| bitstream.checksum.fl_str_mv | 9cc8e1c8b11aaaaa64630a6d977d560a |
| bitstream.checksumAlgorithm.fl_str_mv | MD5 |
| bitstream.url.fl_str_mv | http://repositorio2.unb.br/jspui/bitstream/10482/36477/1/ARTIGO_GenomeWideAnalysis.pdf |
| collection | Repositório Institucional da UnB |
| dc.contributor.author.fl_str_mv | Pereira, Wendell Jacinto Melo, Arthur Tavares de Oliveira Coelho, Alexandre Siqueira Guedes Rodrigues, Fabiana Aparecida Caldart Mamidi, Sujan Alencar, Sérgio Amorim de Lanna, Anna Cristina Valdisser, Paula Arielle Mendes Ribeiro Brondani, Claudio Nascimento Júnior, Ivanildo Ramalho do Borba, Tereza Cristina de Oliveira Vianello, Rosana Pereira |
| dc.date.accessioned.fl_str_mv | 2020-01-24T10:31:10Z |
| dc.date.available.fl_str_mv | 2020-01-24T10:31:10Z |
| dc.date.issued.fl_str_mv | 2019 |
| dc.identifier.citation.fl_str_mv | PEREIRA, Wendell Jacinto et al. Genome-wide analysis of the transcriptional response to drought stress in root and leaf of common bean. Genetics and Molecular Biology, n. 1, e20180259, 2019. DOI: https://doi.org/10.1590/1678-4685-gmb-2018-0259. Disponível em: http://scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019005032102. Acesso em: 01 jul. 2020. |
| dc.identifier.doi.pt_BR.fl_str_mv | https://doi.org/10.1590/1678-4685-gmb-2018-0259 |
| dc.identifier.orcid.none.fl_str_mv | http://orcid.org/0000-0001-6954-6608 http://orcid.org/0000-0003-0644-5652 |
| dc.identifier.uri.fl_str_mv | https://repositorio.unb.br/handle/10482/36477 |
| dc.language.iso.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Sociedade Brasileira de Genética |
| dc.rights.driver.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositório Institucional da UnB instname:Universidade de Brasília (UnB) instacron:UNB |
| dc.subject.keyword.pt_BR.fl_str_mv | Feijão Déficit hídrico Genômica Polimorfismo (Genética) |
| dc.title.pt_BR.fl_str_mv | Genome-wide analysis of the transcriptional response to drought stress in root and leaf of common bean |
| dc.type.driver.fl_str_mv | info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | Genes related to the response to drought stress in leaf and root tissue of drought-susceptible (DS) and tolerant (DT) genotypes were characterized by RNA-Seq. In total, 54,750 transcripts, representative of 28,590 genes, were identified; of these, 1,648 were of high-fidelity (merge of 12 libraries) and described for the first time in the Andean germplasm. From the 1,239 differentially expressed genes (DEGs), 458 were identified in DT, with a predominance of genes in categories of oxidative stress, response to stimulus and kinase activity. Most genes related to oxidation-reduction terms in roots were early triggered in DT (T75) compared to DS (T150) suggestive of a mechanism of tolerance by reducing the damage from ROS. Among the KEGG enriched by DEGs up-regulated in DT leaves, two related to the formation of Sulfur-containing compounds, which are known for their involvement in tolerance to abiotic stresses, were common to all treatments. Through qPCR, 88.64% of the DEGs were validated. A total of 151,283 variants were identified and functional effects estimated for 85,780. The raw data files were submitted to the NCBI database. A transcriptome map revealed new genes and isoforms under drought. These results supports a better understanding of the drought tolerance mechanisms in beans. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | UNB_44d6d5ba1465e2844d4e66ab6b2c16c8 |
| identifier_str_mv | PEREIRA, Wendell Jacinto et al. Genome-wide analysis of the transcriptional response to drought stress in root and leaf of common bean. Genetics and Molecular Biology, n. 1, e20180259, 2019. DOI: https://doi.org/10.1590/1678-4685-gmb-2018-0259. Disponível em: http://scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019005032102. Acesso em: 01 jul. 2020. |
| instacron_str | UNB |
| institution | UNB |
| instname_str | Universidade de Brasília (UnB) |
| language | eng |
| network_acronym_str | UNB |
| network_name_str | Repositório Institucional da UnB |
| oai_identifier_str | oai:repositorio.unb.br:10482/36477 |
| publishDate | 2019 |
| publishDateSort | 2019 |
| publisher.none.fl_str_mv | Sociedade Brasileira de Genética |
| reponame_str | Repositório Institucional da UnB |
| repository.mail.fl_str_mv | repositorio@unb.br |
| repository.name.fl_str_mv | Repositório Institucional da UnB - Universidade de Brasília (UnB) |
| repository_id_str | |
| spelling | Pereira, Wendell JacintoMelo, Arthur Tavares de OliveiraCoelho, Alexandre Siqueira GuedesRodrigues, Fabiana Aparecida CaldartMamidi, SujanAlencar, Sérgio Amorim deLanna, Anna CristinaValdisser, Paula Arielle Mendes RibeiroBrondani, ClaudioNascimento Júnior, Ivanildo Ramalho doBorba, Tereza Cristina de OliveiraVianello, Rosana Pereira2020-01-24T10:31:10Z2020-01-24T10:31:10Z2019PEREIRA, Wendell Jacinto et al. Genome-wide analysis of the transcriptional response to drought stress in root and leaf of common bean. Genetics and Molecular Biology, n. 1, e20180259, 2019. DOI: https://doi.org/10.1590/1678-4685-gmb-2018-0259. Disponível em: http://scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019005032102. Acesso em: 01 jul. 2020.https://repositorio.unb.br/handle/10482/36477https://doi.org/10.1590/1678-4685-gmb-2018-0259http://orcid.org/0000-0001-6954-6608http://orcid.org/0000-0003-0644-5652Sociedade Brasileira de GenéticaLicense information: This is an open-access article distributed under the terms of the Creative Commons Attribution License (type CC-BY), which permits unrestricted use, distribution and reproduction in any medium, provided the original article is properly cited.info:eu-repo/semantics/openAccessGenome-wide analysis of the transcriptional response to drought stress in root and leaf of common beaninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFeijãoDéficit hídricoGenômicaPolimorfismo (Genética)Genes related to the response to drought stress in leaf and root tissue of drought-susceptible (DS) and tolerant (DT) genotypes were characterized by RNA-Seq. In total, 54,750 transcripts, representative of 28,590 genes, were identified; of these, 1,648 were of high-fidelity (merge of 12 libraries) and described for the first time in the Andean germplasm. From the 1,239 differentially expressed genes (DEGs), 458 were identified in DT, with a predominance of genes in categories of oxidative stress, response to stimulus and kinase activity. Most genes related to oxidation-reduction terms in roots were early triggered in DT (T75) compared to DS (T150) suggestive of a mechanism of tolerance by reducing the damage from ROS. Among the KEGG enriched by DEGs up-regulated in DT leaves, two related to the formation of Sulfur-containing compounds, which are known for their involvement in tolerance to abiotic stresses, were common to all treatments. Through qPCR, 88.64% of the DEGs were validated. A total of 151,283 variants were identified and functional effects estimated for 85,780. The raw data files were submitted to the NCBI database. A transcriptome map revealed new genes and isoforms under drought. These results supports a better understanding of the drought tolerance mechanisms in beans.engreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNBORIGINALARTIGO_GenomeWideAnalysis.pdfARTIGO_GenomeWideAnalysis.pdfapplication/pdf2598529http://repositorio2.unb.br/jspui/bitstream/10482/36477/1/ARTIGO_GenomeWideAnalysis.pdf9cc8e1c8b11aaaaa64630a6d977d560aMD51open access10482/364772023-05-24 20:34:25.058open accessoai:repositorio.unb.br:10482/36477Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2023-05-24T23:34:25Repositório Institucional da UnB - Universidade de Brasília (UnB) |
| spellingShingle | Genome-wide analysis of the transcriptional response to drought stress in root and leaf of common bean Pereira, Wendell Jacinto Feijão Déficit hídrico Genômica Polimorfismo (Genética) |
| status_str | publishedVersion |
| title | Genome-wide analysis of the transcriptional response to drought stress in root and leaf of common bean |
| title_full | Genome-wide analysis of the transcriptional response to drought stress in root and leaf of common bean |
| title_fullStr | Genome-wide analysis of the transcriptional response to drought stress in root and leaf of common bean |
| title_full_unstemmed | Genome-wide analysis of the transcriptional response to drought stress in root and leaf of common bean |
| title_short | Genome-wide analysis of the transcriptional response to drought stress in root and leaf of common bean |
| title_sort | Genome-wide analysis of the transcriptional response to drought stress in root and leaf of common bean |
| topic | Feijão Déficit hídrico Genômica Polimorfismo (Genética) |
| url | https://repositorio.unb.br/handle/10482/36477 https://doi.org/10.1590/1678-4685-gmb-2018-0259 http://orcid.org/0000-0001-6954-6608 http://orcid.org/0000-0003-0644-5652 |