Genome-wide analysis of the transcriptional response to drought stress in root and leaf of common bean
Kaydedildi:
| Yazar: | |
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| Yayın Tarihi: | 2019 |
| Diğer Yazarlar: | , , , , , , , , , , |
| Materyal Türü: | Article |
| Dil: | eng |
| Kaynak: | Repositório Institucional da UnB |
| Download full: | https://repositorio.unb.br/handle/10482/36477 https://doi.org/10.1590/1678-4685-gmb-2018-0259 http://orcid.org/0000-0001-6954-6608 http://orcid.org/0000-0003-0644-5652 |
Özet: | Genes related to the response to drought stress in leaf and root tissue of drought-susceptible (DS) and tolerant (DT) genotypes were characterized by RNA-Seq. In total, 54,750 transcripts, representative of 28,590 genes, were identified; of these, 1,648 were of high-fidelity (merge of 12 libraries) and described for the first time in the Andean germplasm. From the 1,239 differentially expressed genes (DEGs), 458 were identified in DT, with a predominance of genes in categories of oxidative stress, response to stimulus and kinase activity. Most genes related to oxidation-reduction terms in roots were early triggered in DT (T75) compared to DS (T150) suggestive of a mechanism of tolerance by reducing the damage from ROS. Among the KEGG enriched by DEGs up-regulated in DT leaves, two related to the formation of Sulfur-containing compounds, which are known for their involvement in tolerance to abiotic stresses, were common to all treatments. Through qPCR, 88.64% of the DEGs were validated. A total of 151,283 variants were identified and functional effects estimated for 85,780. The raw data files were submitted to the NCBI database. A transcriptome map revealed new genes and isoforms under drought. These results supports a better understanding of the drought tolerance mechanisms in beans. |
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